Protein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare research.

Aquaculture is a fast-growing industry and therefore welfare and environmental impact have become of utmost importance. Preventing stress associated to common aquaculture practices and optimizing the fish stress response by quantification of the stress level, are important steps towards the improvement of welfare standards. Stress is characterized by a cascade of physiological responses that, in-turn, induce further changes at the whole-animal level.

These can either increase fitness or impair welfare. Nevertheless, monitorization of this dynamic process has, up until now, relied on indicators that are only a snapshot of the stress level experienced. Promising technological tools, such as proteomics, allow an unbiased approach for the discovery of potential biomarkers for stress monitoring. Within this scope, using Gilthead seabream (Sparus aurata) as a model, three chronic stress conditions, namely overcrowding, handling and hypoxia, were employed to evaluate the potential of the fish protein-based adaptations as reliable signatures of chronic stress, in contrast with the commonly used hormonal and metabolic indicators.

A broad spectrum of biological variation regarding cortisol and glucose levels was observed, the values of which rose higher in net-handled fish. In this sense, a potential pattern of stressor-specificity was clear, as the level of response varied markedly between a persistent (crowding) and a repetitive stressor (handling). Gel-based proteomics analysis of the plasma proteome also revealed that net-handled fish had the highest number of differential proteins, compared to the other trials.

Mass spectrometric analysis, followed by gene ontology enrichment and protein-protein interaction analyses, characterized those as humoral components of the innate immune system and key elements of the response to stimulus.Overall, this study represents the first screening of more reliable signatures of physiological adaptation to chronic stress in fish, allowing the future development of novel biomarker models to monitor fish welfare.

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml

NB319-SBB 30 ml
EUR 369

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW

NB319-BB70 70 ml
EUR 485

DAB

HY-15912 500mg
EUR 108

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04727 5g Ask for price

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04728 25g Ask for price

DAB Tablet

D3225-020 20TB
EUR 142

DAB Tablet

D3225-050 50TB
EUR 246

DAB Chromogen Kit

abx097197-1Kit 1 Kit
EUR 272

H-Dab-OH·HCl

5-04703 5g Ask for price

H-Dab-OH·HCl

5-04704 25g Ask for price

Boc-Dab-OH

5-04711 5g Ask for price

Boc-Dab-OH

5-04712 25g Ask for price

Fmoc-Dab-OAll·HCl

5-04720 5g Ask for price

Fmoc-Dab-OH·HCl

5-04721 5g Ask for price

Fmoc-Dab-OH·HCl

5-04722 25g Ask for price

Fmoc-Dab-OH

5-04735 1g Ask for price

Fmoc-Dab-OH

5-04736 5g Ask for price

DAB Chromogen Concentrate

D3226-002 20ml
EUR 161

DAB Chromogen Concentrate

D3226-005 50ml
EUR 334

Boc-Dab-OH

A-3215.0001 1.0g
EUR 126
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0005 5.0g
EUR 418
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0025 25.0g
EUR 1578
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

DAB Substrate kit

30015 1Kit
EUR 143
Description: Minimum order quantity: 1 unit of 1Kit

Z-Dab-OH

C-3705.0001 1.0g
EUR 115
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0005 5.0g
EUR 273
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0025 25.0g
EUR 998
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

DAB Differentiating Solution

DDS500 500 ml
EUR 109

H-Dab-OH

F-3050.0001 1.0g
EUR 115
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0005 5.0g
EUR 225
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0025 25.0g
EUR 803
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

DAB Chromogen, 4ml

NB314D 4 ml
EUR 246

DL-Dab.2HCl

A6727-100000 100 g
EUR 569
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-25000 25 g
EUR 309
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-5000 5 g
EUR 180
Description: DL-Dab.2HCl

DAB Chromogen Concentrate

ACB030 30 ml
EUR 119

DAB Chromogen Concentrate

ACB060 60 ml
EUR 165

DAB Chromogen Concentrate

ACB125 125 ml
EUR 245

DAB Chromogen Concentrate

ACB500 500 ml
EUR 671

DAB Chromogen Concentrate

ACB999 1000 ml
EUR 1159

DAB Substrate Buffer

ACC500 500 ml
EUR 72

DAB Substrate Buffer

ACC999 1000 ml
EUR 80

DAB Enhancer Solution

ACM030 30 ml
EUR 71

DAB Away Kit

ACP001 500 ml
EUR 98

Fmoc-Dab-OH

B-2300.0001 1.0g
EUR 151
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

Fmoc-Dab-OH

B-2300.0005 5.0g
EUR 515
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

HiQ Block for IHC

B3077-010 100ml
EUR 134

HiQ Block for IHC

B3077-050 500ml
EUR 263

Enhancing Wash Buffer, For rinse of IHC-DAB & AEC stains and fluorescence, 4 Liters; Ready-To-Use

NB301 4 Liters
EUR 473

Enhancing Wash Buffer, For rinse of IHC-DAB & AEC stains and fluorescence, 1 liter of 5X; Concentrate

NB301-5C 1 Liter of 5X
EUR 508

Enhancing Wash Buffer, For rinse of IHC-DAB & AEC stains and fluorescence, 1 Liter; Ready-To-Use

NB301S 1 Liter
EUR 218

IHC Enzyme Antigen Retrieval Reagent

AR0022 50mL (for 500-750 assays)
EUR 106

BCIP/NBT Solution for IHC

B3007-005 50ml
EUR 134

BCIP/NBT Solution for IHC

B3007-010 100ml
EUR 188

BCIP/NBT Solution for IHC

B3007-050 500ml
EUR 475

BCIP-NBT Dye for IHC

G447 250 ml
EUR 254

UltraBrite? Red IHC chromogen (AP)

M1305-30
EUR 349

UltraBrite? Blue IHC chromogen (AP)

M1307-30
EUR 370

UltraBrite? Blue IHC chromogen (HRP)

M1308-30
EUR 457

UltraBrite? Green IHC chromogen (AP)

M1309-30
EUR 425

UltraBrite? Yellow IHC chromogen (HRP)

M1310-30
EUR 457

UltraBrite? Black IHC chromogen (HRP)

M1311-30
EUR 457

UltraBrite? Red IHC chromogen (HRP)

M1312-30
EUR 370

Universal IHC Diluent/Blocker/Stabilizer

85R-1021 1 liter
EUR 692
Description: Universal Diluent/Blocker/Stabilizer for use in IHC

EMA antibody (Prediluted for IHC)

75R-1002 7 ml
EUR 225
Description: Mouse monoclonal EMA antibody (Prediluted for IHC)

hCG antibody (Prediluted for IHC)

75R-1005 7 ml
EUR 244
Description: Mouse monoclonal hCG antibody (Prediluted for IHC)

BRDU antibody (Prediluted for IHC)

75R-1006 7 ml
EUR 225
Description: Mouse monoclonal BRDU antibody (Prediluted for IHC)

EpCAM antibody (Prediluted for IHC)

75R-1014 7 ml
EUR 225
Description: Mouse monoclonal EpCAM antibody (Prediluted for IHC)

p53 antibody (Prediluted for IHC)

75R-1015 7 ml
EUR 225
Description: Mouse monoclonal p53 antibody (Prediluted for IHC)

PCNA antibody (Prediluted for IHC)

75R-1016 7 ml
EUR 225
Description: Mouse monoclonal PCNA antibody (Prediluted for IHC)

S100 antibody (Prediluted for IHC)

75R-1017 7 ml
EUR 225
Description: Mouse monoclonal S100 antibody (Prediluted for IHC)

TSH antibody (Prediluted for IHC)

75R-1019 7 ml
EUR 225
Description: Mouse monoclonal TSH antibody (Prediluted for IHC)

Thyroglobulin antibody (Prediluted for IHC)

75R-1020 7 ml
EUR 225
Description: Mouse monoclonal Thyroglobulin antibody (Prediluted for IHC)

NSE antibody (Prediluted for IHC)

75R-1023 7 ml
EUR 232
Description: Mouse monoclonal NSE antibody (Prediluted for IHC)

Ferritin antibody (Prediluted for IHC)

75R-1026 7 ml
EUR 187
Description: Rabbit polyclonal Ferritin antibody (Prediluted for IHC)

Vimmentin antibody (Prediluted for IHC)

75R-1027 7 ml
EUR 187
Description: Rabbit polyclonal Vimmentin antibody (Prediluted for IHC)

Fibronectin antibody (Prediluted for IHC)

75R-1028 7 ml
EUR 187
Description: Rabbit polyclonal Fibronectin antibody (Prediluted for IHC)

CRP antibody (Prediluted for IHC)

75R-1029 7 ml
EUR 187
Description: Rabbit polyclonal CRP antibody (Prediluted for IHC)

CEA antibody (Prediluted for IHC)

75R-1030 7 ml
EUR 187
Description: Rabbit polyclonal CEA antibody (Prediluted for IHC)

HBcAg antibody (Prediluted for IHC)

75R-1031 7 ml
EUR 187
Description: Rabbit polyclonal HBcAg antibody (Prediluted for IHC)

Calcitonin antibody (Prediluted for IHC)

75R-1032 7 ml
EUR 187
Description: Rabbit polyclonal Calcitonin antibody (Prediluted for IHC)

Synaptophysin antibody (Prediluted for IHC)

75R-1033 7 ml
EUR 187
Description: Rabbit polyclonal Synaptophysin antibody (Prediluted for IHC)

Thyroglobulin antibody (Prediluted for IHC)

75R-1034 7 ml
EUR 187
Description: Rabbit polyclonal Thyroglobulin antibody (Prediluted for IHC)

PSA antibody (Prediluted for IHC)

75R-1035 7 ml
EUR 187
Description: Rabbit polyclonal PSA antibody (Prediluted for IHC)

Ubiquitin antibody (Prediluted for IHC)

75R-1036 7 ml
EUR 187
Description: Rabbit polyclonal Ubiquitin antibody (Prediluted for IHC)

Insulin antibody (Prediluted for IHC)

75R-1037 7 ml
EUR 187
Description: Rabbit polyclonal Insulin antibody (Prediluted for IHC)

MUC2 antibody (Prediluted for IHC)

75R-1038 7 ml
EUR 187
Description: Rabbit polyclonal MUC2 antibody (Prediluted for IHC)

S100 antibody (Prediluted for IHC)

75R-1039 7 ml
EUR 187
Description: Rabbit polyclonal S100 Protein antibody (Prediluted for IHC)

MBP antibody (Prediluted for IHC)

75R-1040 7 ml
EUR 187
Description: Rabbit polyclonal MBP antibody (Prediluted for IHC)

GFAP antibody (Prediluted for IHC)

75R-1041 7 ml
EUR 187
Description: Rabbit polyclonal GFAP antibody (Prediluted for IHC)

AFP antibody (Prediluted for IHC)

75R-1042 7 ml
EUR 187
Description: Rabbit polyclonal AFP antibody (Prediluted for IHC)

Lysozyme antibody (Prediluted for IHC)

75R-1043 7 ml
EUR 187
Description: Rabbit polyclonal Lysozyme antibody (Prediluted for IHC)

Transferrin antibody (Prediluted for IHC)

75R-1047 7 ml
EUR 187
Description: Rabbit polyclonal Transferrin antibody

Desmin antibody (Prediluted for IHC)

75R-1048 7 ml
EUR 187
Description: Rabbit polyclonal Desmin antibody (Prediluted for IHC)

MPO antibody (Prediluted for IHC)

75R-1050 7 ml
EUR 187
Description: Rabbit polyclonal MPO antibody (Prediluted for IHC)

Insulin antibody (Prediluted for IHC)

75R-1058 7 ml
EUR 187
Description: Guinea pig polyclonal Insulin antibody (Prediluted for IHC)

H-Dab(Z)-OH

5-04707 5g Ask for price
Scroll to Top