Protein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare research.

Aquaculture is a fast-growing industry and therefore welfare and environmental impact have become of utmost importance. Preventing stress associated to common aquaculture practices and optimizing the fish stress response by quantification of the stress level, are important steps towards the improvement of welfare standards. Stress is characterized by a cascade of physiological responses that, in-turn, induce further changes at the whole-animal level.

These can either increase fitness or impair welfare. Nevertheless, monitorization of this dynamic process has, up until now, relied on indicators that are only a snapshot of the stress level experienced. Promising technological tools, such as proteomics, allow an unbiased approach for the discovery of potential biomarkers for stress monitoring. Within this scope, using Gilthead seabream (Sparus aurata) as a model, three chronic stress conditions, namely overcrowding, handling and hypoxia, were employed to evaluate the potential of the fish protein-based adaptations as reliable signatures of chronic stress, in contrast with the commonly used hormonal and metabolic indicators.

A broad spectrum of biological variation regarding cortisol and glucose levels was observed, the values of which rose higher in net-handled fish. In this sense, a potential pattern of stressor-specificity was clear, as the level of response varied markedly between a persistent (crowding) and a repetitive stressor (handling). Gel-based proteomics analysis of the plasma proteome also revealed that net-handled fish had the highest number of differential proteins, compared to the other trials.

Mass spectrometric analysis, followed by gene ontology enrichment and protein-protein interaction analyses, characterized those as humoral components of the innate immune system and key elements of the response to stimulus.Overall, this study represents the first screening of more reliable signatures of physiological adaptation to chronic stress in fish, allowing the future development of novel biomarker models to monitor fish welfare.

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml

NB319-SBB 30 ml
EUR 442.8

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW

NB319-BB70 70 ml
EUR 582

DAB

HY-15912 500mg
EUR 129.6

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04727 5g Ask for price

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04728 25g Ask for price

DAB Tablet

D3225-020 20TB
EUR 170.4

DAB Tablet

D3225-050 50TB
EUR 295.2

H-Dab-OH·HCl

5-04703 5g Ask for price

H-Dab-OH·HCl

5-04704 25g Ask for price

Boc-Dab-OH

5-04711 5g Ask for price

Boc-Dab-OH

5-04712 25g Ask for price

Fmoc-Dab-OAll·HCl

5-04720 5g Ask for price

Fmoc-Dab-OH·HCl

5-04721 5g Ask for price

Fmoc-Dab-OH·HCl

5-04722 25g Ask for price

Fmoc-Dab-OH

5-04735 1g Ask for price

Fmoc-Dab-OH

5-04736 5g Ask for price

DAB Substrate kit

30015 1Kit
EUR 171.6
Description: Minimum order quantity: 1 unit of 1Kit

DAB Chromogen Kit

abx097197-1Kit 1 Kit
EUR 326.4

DL-Dab.2HCl

A6727-100000 100 g
EUR 682.8
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-25000 25 g
EUR 370.8
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-5000 5 g
EUR 216
Description: DL-Dab.2HCl

Boc-Dab-OH

A-3215.0001 1.0g
EUR 151.2
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0005 5.0g
EUR 501.6
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0025 25.0g
EUR 1893.6
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Fmoc-Dab-OH

B-2300.0001 1.0g
EUR 181.2
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

Fmoc-Dab-OH

B-2300.0005 5.0g
EUR 618
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

DAB Chromogen Concentrate

D3226-002 20ml
EUR 193.2

DAB Chromogen Concentrate

D3226-005 50ml
EUR 400.8

DAB Differentiating Solution

DDS500 500 ml
EUR 130.8

Z-Dab-OH

C-3705.0001 1.0g
EUR 138
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0005 5.0g
EUR 327.6
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0025 25.0g
EUR 1197.6
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

DAB Chromogen Concentrate

ACB030 30 ml
EUR 142.8

DAB Chromogen Concentrate

ACB060 60 ml
EUR 198

DAB Chromogen Concentrate

ACB125 125 ml
EUR 294

DAB Chromogen Concentrate

ACB500 500 ml
EUR 805.2

DAB Chromogen Concentrate

ACB999 1000 ml
EUR 1390.8

DAB Substrate Buffer

ACC500 500 ml
EUR 86.4

DAB Substrate Buffer

ACC999 1000 ml
EUR 96

DAB Enhancer Solution

ACM030 30 ml
EUR 85.2

DAB Away Kit

ACP001 500 ml
EUR 117.6

DAB Chromogen, 4ml

NB314D 4 ml
EUR 295.2

H-Dab-OH

F-3050.0001 1.0g
EUR 138
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0005 5.0g
EUR 270
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0025 25.0g
EUR 963.6
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

HiQ Block for IHC

B3077-010 100ml
EUR 160.8

HiQ Block for IHC

B3077-050 500ml
EUR 315.6

Enhancing Wash Buffer, For rinse of IHC-DAB & AEC stains and fluorescence, 4 Liters; Ready-To-Use

NB301 4 Liters
EUR 567.6

Enhancing Wash Buffer, For rinse of IHC-DAB & AEC stains and fluorescence, 1 liter of 5X; Concentrate

NB301-5C 1 Liter of 5X
EUR 609.6

Enhancing Wash Buffer, For rinse of IHC-DAB & AEC stains and fluorescence, 1 Liter; Ready-To-Use

NB301S 1 Liter
EUR 261.6

H-Dab(Z)-OH

5-04707 5g Ask for price

H-Dab(Z)-OH

5-04708 25g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04713 1g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04714 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04715 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04716 25g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04717 5g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04718 25g Ask for price

Fmoc-Dab(Ac)-OH

5-04723 5g Ask for price

Fmoc-Dab(Ac)-OH

5-04724 25g Ask for price

Fmoc-Dab(Boc)-OH

5-04725 5g Ask for price

Fmoc-Dab(Boc)-OH

5-04726 25g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04729 1g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04730 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04731 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04732 25g Ask for price

Fmoc-Dab(Mtt)-OH

5-04733 1g Ask for price

Fmoc-Dab(Mtt)-OH

5-04734 5g Ask for price

Fmoc-Dab(Dde)-OH

5-04739 1g Ask for price

Fmoc-Dab(Dde)-OH

5-04740 5g Ask for price

Fmoc-Dab(ivDde)-OH

5-04741 1g Ask for price

Fmoc-Dab(ivDde)-OH

5-04742 5g Ask for price

Fmoc-Dab(Z)-OH

5-04743 1g Ask for price

Fmoc-Dab(Z)-OH

5-04744 5g Ask for price

Boc-D-Dab-OH

5-04745 1g Ask for price

Boc-D-Dab-OH

5-04746 5g Ask for price

Fmoc-D-Dab-OH

5-04751 1g Ask for price

Fmoc-D-Dab-OH

5-04752 5g Ask for price

10-DAB (10-Deacetylbaccatin)

A4395-25 25 mg
EUR 616.8
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-5 5 mg
EUR 199.2
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-5.1 10 mM (in 1mL DMSO)
EUR 351.6
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-S Evaluation Sample
EUR 97.2
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

Fmoc-Dab(Fmoc)-Oh

abx188721-250g 250 g
EUR 2064

H-Dab(Boc)-OH

A-3305.0001 1.0g
EUR 240
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

H-Dab(Boc)-OH

A-3305.0005 5.0g
EUR 835.2
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

Boc-Dab(Fmoc)-OH

A-3520.0001 1.0g
EUR 270
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Fmoc)-OH

A-3520.0005 5.0g
EUR 936
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Aloc)-OH

A-4125.0001 1.0g
EUR 370.8
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-Dab(Aloc)-OH

A-4125.0005 5.0g
EUR 1371.6
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-D-Dab-OH

A-4215.0001 1.0g
EUR 501.6
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-D-Dab-OH

A-4215.0005 5.0g
EUR 1864.8
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-Dab-OtBu · HCl

A-4415.0001 1.0g
EUR 370.8
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Boc-Dab-OtBu · HCl

A-4415.0005 5.0g
EUR 1371.6
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Fmoc-Dab(Boc)-OH

B-1800.0001 1.0g
EUR 240
Description: Sum Formula: C24H28N2O6; CAS# [125238-99-5]

Fmoc-Dab(Boc)-OH

B-1800.0005 5.0g
EUR 819.6
Description: Sum Formula: C24H28N2O6; CAS# [125238-99-5]

Fmoc-Dab(Fmoc)-OH

B-2270.0001 1.0g
EUR 283.2
Description: Sum Formula: C34H30N2O6; CAS# [201473-83-8]

Fmoc-Dab(Fmoc)-OH

B-2270.0005 5.0g
EUR 1039.2
Description: Sum Formula: C34H30N2O6; CAS# [201473-83-8]

Fmoc-D-Dab-OH

B-2365.0001 1.0g
EUR 501.6
Description: Sum Formula: C19H20N2O4; CAS# [201484-12-0]

Fmoc-D-Dab-OH

B-2365.0005 5.0g
EUR 1864.8
Description: Sum Formula: C19H20N2O4; CAS# [201484-12-0]

Fmoc-Dab(Aloc)-OH

B-2850.0001 1.0g
EUR 356.4
Description: Sum Formula: C23H24N2O6; CAS# [204316-32-5]

Fmoc-Dab(Aloc)-OH

B-2850.0005 5.0g
EUR 1327.2
Description: Sum Formula: C23H24N2O6; CAS# [204316-32-5]

Fmoc-Dab(Z)-OH

B-3250.0001 1.0g
EUR 313.2
Description: Sum Formula: C27H26N2O6; CAS# [252049-08-4]

Fmoc-Dab(Z)-OH

B-3250.0005 5.0g
EUR 1110
Description: Sum Formula: C27H26N2O6; CAS# [252049-08-4]

Fmoc-Dab(ivDde)-OH

B-4065.0001 1.0g
EUR 356.4
Description: Sum Formula: C32H38N2O6; CAS# [607366-21-2]

Fmoc-Dab(ivDde)-OH

B-4065.0005 5.0g
EUR 1311.6
Description: Sum Formula: C32H38N2O6; CAS# [607366-21-2]

DAB (Post) Enhancing Solution

DES500 500 ml
EUR 105.6

DAB (Post) Enhancing Solution

DES999 1000 ml
EUR 130.8

Z-Dab(Z)-OH

C-3690.0001 1.0g
EUR 240
Description: Sum Formula: C20H22N2O6; CAS# [55478-23-4]

Z-Dab(Z)-OH

C-3690.0005 5.0g
EUR 835.2
Description: Sum Formula: C20H22N2O6; CAS# [55478-23-4]

Z-D-Dab-OH

C-3770.0001 1.0g
EUR 270
Description: Sum Formula: C12H16N2O4; CAS# [70882-66-5]

Z-D-Dab-OH

C-3770.0005 5.0g
EUR 950.4
Description: Sum Formula: C12H16N2O4; CAS# [70882-66-5]

DAB Chromogen/Substrate Kit

ACH500 500 Slides
EUR 102

DAB Substrate (High Contrast)

ACU250 250 ml
EUR 102

DAB Substrate (High Contrast)

ACU500 500 ml
EUR 117.6

DAB Substrate (High Contrast)

ACU999 1000 ml
EUR 142.8

DAB Substrate buffer, 70ml

NB314SB 70 ml
EUR 331.2

Ac-Dab(Boc)-OH

F-1020.0250 250.0mg
EUR 283.2
Description: Sum Formula: C11H20N2O5; CAS# [201351-32-8]

Ac-Dab(Boc)-OH

F-1020.1000 1.0g
EUR 792
Description: Sum Formula: C11H20N2O5; CAS# [201351-32-8]

H-D-Dab-OH

F-3055.0001 1.0g
EUR 270
Description: Sum Formula: C4H10N2O2; CAS# [26908-94-1] net

H-D-Dab-OH

F-3055.0005 5.0g
EUR 950.4
Description: Sum Formula: C4H10N2O2; CAS# [26908-94-1] net

Sensitive DAB Stain Kit

PW023 5Preps, 5prep
EUR 82.97

Universal IHC Diluent/Blocker/Stabilizer

85R-1021 1 liter
EUR 830.4
Description: Universal Diluent/Blocker/Stabilizer for use in IHC

EMA antibody (Prediluted for IHC)

75R-1002 7 ml
EUR 270
Description: Mouse monoclonal EMA antibody (Prediluted for IHC)

hCG antibody (Prediluted for IHC)

75R-1005 7 ml
EUR 292.8
Description: Mouse monoclonal hCG antibody (Prediluted for IHC)

BRDU antibody (Prediluted for IHC)

75R-1006 7 ml
EUR 270
Description: Mouse monoclonal BRDU antibody (Prediluted for IHC)

EpCAM antibody (Prediluted for IHC)

75R-1014 7 ml
EUR 270
Description: Mouse monoclonal EpCAM antibody (Prediluted for IHC)

p53 antibody (Prediluted for IHC)

75R-1015 7 ml
EUR 270
Description: Mouse monoclonal p53 antibody (Prediluted for IHC)

PCNA antibody (Prediluted for IHC)

75R-1016 7 ml
EUR 270
Description: Mouse monoclonal PCNA antibody (Prediluted for IHC)

S100 antibody (Prediluted for IHC)

75R-1017 7 ml
EUR 270
Description: Mouse monoclonal S100 antibody (Prediluted for IHC)

TSH antibody (Prediluted for IHC)

75R-1019 7 ml
EUR 270
Description: Mouse monoclonal TSH antibody (Prediluted for IHC)

Thyroglobulin antibody (Prediluted for IHC)

75R-1020 7 ml
EUR 270
Description: Mouse monoclonal Thyroglobulin antibody (Prediluted for IHC)

NSE antibody (Prediluted for IHC)

75R-1023 7 ml
EUR 278.4
Description: Mouse monoclonal NSE antibody (Prediluted for IHC)

Ferritin antibody (Prediluted for IHC)

75R-1026 7 ml
EUR 224.4
Description: Rabbit polyclonal Ferritin antibody (Prediluted for IHC)

Vimmentin antibody (Prediluted for IHC)

75R-1027 7 ml
EUR 224.4
Description: Rabbit polyclonal Vimmentin antibody (Prediluted for IHC)

Fibronectin antibody (Prediluted for IHC)

75R-1028 7 ml
EUR 224.4
Description: Rabbit polyclonal Fibronectin antibody (Prediluted for IHC)

CRP antibody (Prediluted for IHC)

75R-1029 7 ml
EUR 224.4
Description: Rabbit polyclonal CRP antibody (Prediluted for IHC)

CEA antibody (Prediluted for IHC)

75R-1030 7 ml
EUR 224.4
Description: Rabbit polyclonal CEA antibody (Prediluted for IHC)

HBcAg antibody (Prediluted for IHC)

75R-1031 7 ml
EUR 224.4
Description: Rabbit polyclonal HBcAg antibody (Prediluted for IHC)

Calcitonin antibody (Prediluted for IHC)

75R-1032 7 ml
EUR 224.4
Description: Rabbit polyclonal Calcitonin antibody (Prediluted for IHC)

Synaptophysin antibody (Prediluted for IHC)

75R-1033 7 ml
EUR 224.4
Description: Rabbit polyclonal Synaptophysin antibody (Prediluted for IHC)

Thyroglobulin antibody (Prediluted for IHC)

75R-1034 7 ml
EUR 224.4
Description: Rabbit polyclonal Thyroglobulin antibody (Prediluted for IHC)

PSA antibody (Prediluted for IHC)

75R-1035 7 ml
EUR 224.4
Description: Rabbit polyclonal PSA antibody (Prediluted for IHC)

Ubiquitin antibody (Prediluted for IHC)

75R-1036 7 ml
EUR 224.4
Description: Rabbit polyclonal Ubiquitin antibody (Prediluted for IHC)

Insulin antibody (Prediluted for IHC)

75R-1037 7 ml
EUR 224.4
Description: Rabbit polyclonal Insulin antibody (Prediluted for IHC)

MUC2 antibody (Prediluted for IHC)

75R-1038 7 ml
EUR 224.4
Description: Rabbit polyclonal MUC2 antibody (Prediluted for IHC)

S100 antibody (Prediluted for IHC)

75R-1039 7 ml
EUR 224.4
Description: Rabbit polyclonal S100 Protein antibody (Prediluted for IHC)

MBP antibody (Prediluted for IHC)

75R-1040 7 ml
EUR 224.4
Description: Rabbit polyclonal MBP antibody (Prediluted for IHC)

GFAP antibody (Prediluted for IHC)

75R-1041 7 ml
EUR 224.4
Description: Rabbit polyclonal GFAP antibody (Prediluted for IHC)

AFP antibody (Prediluted for IHC)

75R-1042 7 ml
EUR 224.4
Description: Rabbit polyclonal AFP antibody (Prediluted for IHC)

Lysozyme antibody (Prediluted for IHC)

75R-1043 7 ml
EUR 224.4
Description: Rabbit polyclonal Lysozyme antibody (Prediluted for IHC)

Transferrin antibody (Prediluted for IHC)

75R-1047 7 ml
EUR 224.4
Description: Rabbit polyclonal Transferrin antibody

Desmin antibody (Prediluted for IHC)

75R-1048 7 ml
EUR 224.4
Description: Rabbit polyclonal Desmin antibody (Prediluted for IHC)

MPO antibody (Prediluted for IHC)

75R-1050 7 ml
EUR 224.4
Description: Rabbit polyclonal MPO antibody (Prediluted for IHC)

Insulin antibody (Prediluted for IHC)

75R-1058 7 ml
EUR 224.4
Description: Guinea pig polyclonal Insulin antibody (Prediluted for IHC)

BCIP/NBT Solution for IHC

B3007-005 50ml
EUR 160.8
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