Protein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare research.

Aquaculture is a fast-growing industry and therefore welfare and environmental impact have become of utmost importance. Preventing stress associated to common aquaculture practices and optimizing the fish stress response by quantification of the stress level, are important steps towards the improvement of welfare standards. Stress is characterized by a cascade of physiological responses that, in-turn, induce further changes at the whole-animal level.

These can either increase fitness or impair welfare. Nevertheless, monitorization of this dynamic process has, up until now, relied on indicators that are only a snapshot of the stress level experienced. Promising technological tools, such as proteomics, allow an unbiased approach for the discovery of potential biomarkers for stress monitoring. Within this scope, using Gilthead seabream (Sparus aurata) as a model, three chronic stress conditions, namely overcrowding, handling and hypoxia, were employed to evaluate the potential of the fish protein-based adaptations as reliable signatures of chronic stress, in contrast with the commonly used hormonal and metabolic indicators.

A broad spectrum of biological variation regarding cortisol and glucose levels was observed, the values of which rose higher in net-handled fish. In this sense, a potential pattern of stressor-specificity was clear, as the level of response varied markedly between a persistent (crowding) and a repetitive stressor (handling). Gel-based proteomics analysis of the plasma proteome also revealed that net-handled fish had the highest number of differential proteins, compared to the other trials.

Mass spectrometric analysis, followed by gene ontology enrichment and protein-protein interaction analyses, characterized those as humoral components of the innate immune system and key elements of the response to stimulus.Overall, this study represents the first screening of more reliable signatures of physiological adaptation to chronic stress in fish, allowing the future development of novel biomarker models to monitor fish welfare.

Klear Mouse HRP for DAB Bulk Kit 60 ml

D52-60 -
EUR 950

Polink-2 Plus HRP Goat with DAB 18ml Kit

D43-18 -
EUR 385

Polink-2 Plus HRP Mouse with DAB 18ml Kit

D37-18 -
EUR 356

Klear Human HRP-Polymer with DAB Kit 110 ml

D103-110D -
EUR 4065

Klear Human HRP-Polymer with DAB Kit 18 ml

D103-18 -
EUR 795

Polink-2 Plus HRP Human IgG w DAB 18 ml

D88-18 -
EUR 525

Polink-2 Plus HRP Goat for DAB Bulk 110 ml

D43-110 -
EUR 865

Polink-2 Plus HRP Rabbit with DAB with 18ml Kit

D39-18 -
EUR 358

Polink-2 Plus HRP Mouse for DAB Bulk Kit 110 ml

D37-110 -
EUR 953

Polink-2 Plus HRP Sheep IgG with DAB Kit 18 ml

D85-18 -
EUR 385

Polink-2 Plus HRP Human IgG for DAB Bulk 60 ml

D88-60 -
EUR 845

Polink-2 Plus HRP Rabbit for DAB Bulk Kit 110 ml

D39-110 -
EUR 953

Polink-2 Plus HRP Guinea Pig with DAB Kit 18 ml

D83-18 -
EUR 385

Polink-2 Plus HRP Chicken IgY with DAB Kit 18 ml

D84-18 -
EUR 385

Polink-2 Plus HRP Armenian Hamster with DAB Kit 18 ml

D87-18 -
EUR 385

CD3 IHC Antibody

IW-MA1015 -
EUR 285

p53 IHC Antibody

IW-MA1078 -
EUR 285

Tau IHC Antibody

IW-MA1093 -
EUR 285

MBP IHC Antibody

IW-PA1050 -
EUR 285

NSE IHC Antibody

IW-PA1061 -
EUR 285

GFAP IHC Antibody

IW-MA1045 -
EUR 285

PCNA IHC Antibody

IW-MA1083 -
EUR 285

ANG2 IHC Antibody

IW-PA1005 -
EUR 285

CD22 IHC Antibody

IW-PA1018 -
EUR 285

CD44 IHC Antibody

IW-PA1021 -
EUR 285

COX1 IHC Antibody

IW-PA1027 -
EUR 285

PP2A IHC Antibody

IW-PA1068 -
EUR 285

RAGE IHC Antibody

IW-PA1069 -
EUR 285

VEGF IHC Antibody

IW-PA1080 -
EUR 285

MMP2 IHC Antibody

IW-PA1122 -
EUR 285

COX2 IHC Antibody

IW-PA1211 -
EUR 285

FGF8 IHC Antibody

IW-PA1216 -
EUR 285

NF200 IHC Antibody

IW-MA1071 -
EUR 285

CD40L IHC Antibody

IW-PA1020 -
EUR 285

HSP40 IHC Antibody

IW-PA1043 -
EUR 285

HSP60 IHC Antibody

IW-PA1106 -
EUR 285

MMP16 IHC Antibody

IW-PA1123 -
EUR 285

OPCML IHC Antibody

IW-PA1131 -
EUR 285

TNFR2 IHC Antibody

IW-PA1243 -
EUR 285

APAF1 IHC Antibody

IW-PA1249 -
EUR 285

Desmin IHC Antibody

IW-MA1036 -
EUR 285

IFITM1 IHC Antibody

IW-PA1112 -
EUR 285

Elastin IHC Antibody

IW-MA1038 -
EUR 285

Insulin IHC Antibody

IW-MA1052 -
EUR 285

TNFaIP1 IHC Antibody

IW-PA1305 -
EUR 285

Glucagon IHC Antibody

IW-MA1047 -
EUR 285

Vimentin IHC Antibody

IW-MA1102 -
EUR 285

Calretinin IHC Antibody

IW-PA1015 -
EUR 285

Fibronectin IHC Antibody

IW-MA1116 -
EUR 285

Polink-2 Plus HRP Rat with DAB 18ml Kit (No cross to mouse)

D46-18 -
EUR 385

Bcl-2 IHC Antibody

IW-MA1004 -
EUR 285

ABI-1 IHC Antibody

IW-PA1001 -
EUR 285

FAS-L IHC Antibody

IW-PA1101 -
EUR 285

SHP-2 IHC Antibody

IW-PA1114 -
EUR 285

Lamin B1 IHC Antibody

IW-PA1048 -
EUR 285

CD95/PAS IHC Antibody

IW-PA1119 -
EUR 285

TFPI-2 IHC Antibody

IW-PA1248 -
EUR 285

PKC alpha IHC Antibody

IW-PA1065 -
EUR 285

Annexin A1 IHC Antibody

IW-PA1006 -
EUR 285

alpha-SMA IHC Antibody

IW-MA1106 -
EUR 285

Connexin 43 IHC Antibody

IW-PA1026 -
EUR 285

TNF-alpha IHC Antibody

IW-PA1079 -
EUR 285

CBFA1/RUNX2 IHC Antibody

IW-PA1224 -
EUR 285

beta-Actin IHC Antibody

IW-MA1115 -
EUR 285

Cytochrome C IHC Antibody

IW-PA1118 -
EUR 285

Polink-2 Plus HRP Mouse-NR with DAB 18ml Kit (No cross to rat)

D58-18 -
EUR 385

pan Cytokeratin IHC Antibody

IW-MA1081 -
EUR 285

alpha-Tubulin IHC Antibody

IW-MA1107 -
EUR 285

Orexin Receptor IHC Antibody

IW-PA1100 -
EUR 285

Neurotrophin-3 IHC Antibody

IW-PA1062 -
EUR 285

MPO (Myeloperoxidase) IHC Antibody

IW-PA1054 -
EUR 285

Polink-2 Plus HRP Rat for DAB Bulk Kit 110 ml (No cross to mouse)

D46-110 -
EUR 865

IHC-Tek Epitope Retrieval Solution, Ready To Use, 500 ml IHC World: IW-1100

IW-1100 -
EUR 125

AKT 1/2 IHC Antibody

IW-PA1301 -
EUR 285

MAPK 1/3 IHC Antibody

IW-PA1049 -
EUR 285

Lamin A/C IHC Antibody

IW-PA1103 -
EUR 285

HIF-1 alpha IHC Antibody

IW-PA1041 -
EUR 285

CD54 (ICAM-1) IHC Antibody

IW-PA1110 -
EUR 285

HIF-2 alpha IHC Antibody

IW-PA1129 -
EUR 285

Collagen Type IV IHC Antibody

IW-MA1030 -
EUR 285

Polink-2 Plus HRP for DAB Mouse-NR Bulk Kit 110 ml (No cross to rat)

D58-110 -
EUR 865

AQP1 (Aquaporin 1) IHC Antibody

IW-PA1010 -
EUR 285

SHH (Sonic Hedgehog) IHC Antibody

IW-PA1072-1 -
EUR 285

beta Amyloid Protein IHC Antibody

IW-MA1109 -
EUR 285

Apoptosis Inhibitor 5 IHC Antibody

IW-PA1009 -
EUR 285

SCG3 (Secretogranin-3) IHC Antibody

IW-PA1071 -
EUR 285

TH (Tyrosine hydroxylase) IHC Antibody

IW-MA1100 -
EUR 285

ITGA1 (Integrin alpha 1) IHC Antibody

IW-PA1045-1 -
EUR 285

IHC-Tek Epitope Retrieval Steamer Set

IW-1102 -
EUR 525

Polink DS-GM-Hu A Kit for Goat and Mouse antibody on human tissue with DAB & Permanent Red

DS207A-6 -
EUR 515

Polink DS-MRt-Ms A Kit for mouse and Rat antibody on mouse tissue with DAB & Permanent Red

DS210A-6 -
EUR 535

Polink DS-MM-Hu A Kit for 2 mouse antibodies on human tissue with DAB & GBI-Permanent Red

DS203A-6 -
EUR 525

Polink DS-MM-Ms A Kit for 2 mouse antibodies on rodent tissue with DAB & GBI-Permanent Red

DS212A-6 -
EUR 535

IHC-Tek Bone Decalcification Solution, 1000 ml

IW-1900 -
EUR 125

APP (amyloid beta precursor protein) IHC Antibody

IW-PA1362 -
EUR 285

Polink DS-MRt-Hu A Kit for mouse and Rat antibody on human tissue with DAB & GBI-Permanent Red

DS209A-6 -
EUR 525

Polink DS-MR-Hu A1 Kit for mouse and rabbit antibody on human tissue with DAB & GBI-Permanent Red

DS201A-6 -
EUR 485

Polink DS-MR-Hu A2 Kit for mouse and rabit antibody on human tissue with DAB & GBI-Permanent Red

DS202A-6 -
EUR 465

Polink DS-MR-Ms A Kit for mouse and rabbit antibody on mouse tissue with DAB & GBI-Permanent Red

DS233A-6 -
EUR 525

IHC-Tek AEC Peroxidase Substrate Kit - 120 ml

IW-1601 -
EUR 125

Polink DS-GR-Hu/Ms A Kit for goat and rabbit antibody on human/rodent tissue with DAB & Permanent Red

DS205A-6 -
EUR 515

Polink DS-RRt-Hu/Ms A Kit for rabbit and Rat antibody on human & mouse tissue with DAB & Permanent Red

DS211A-6 -
EUR 525

Polink DS-RR-Hu/Ms A Kit for 2 rabbit antibodies on human/rodent tissue with DAB & GBI-Permanent Red

DS204A-6 -
EUR 525

SOCS1 (Suppressor of cytokine signaling 1) IHC Antibody

IW-PA1074 -
EUR 285

Polink DS-GRt-Hu/Ms A Kit for Goat and Rat antibody on human/mouse tissue with DAB & GBI-Permanent Red

DS206A-6 -
EUR 515

Super PAP Pen Liquid Blocker for IHC, small, each

SPM0928 -
EUR 75

Super PAP Pen Liquid Blocker for IHC, large, each

SPR0905 -
EUR 95

IHC-Tek AEC Chromogen Substrate Single Component 50 ml

IW-1603 -
EUR 85

SKP2 (S-phase kinase-associated protein 2) IHC Antibody

IW-PA1102 -
EUR 285

IHC-Tek Methyl Green Solution, Ready To Use, 250 ml

IW-1402 -
EUR 125

IHC-Tek Proteinase K Solution, Ready To Use, 20 ml

IW-1101 -
EUR 185

IHC-Tek PI Counterstain Solution, Ready To Use, 50 ml

IW-1403 -
EUR 58

IHC-Tek Epitope Retrieval Solution, Ready To Use, 1000 ml

IW-1100-1L -
EUR 185

IHC-Tek Mayer's Hematoxylin Solution, Ready To Use, 250 ml

IW-1400 -
EUR 125

IHC-Tek DAPI Counterstain Solution, Ready To Use, 50 ml

IW-1404 -
EUR 65

IHC-Tek Peroxidase Blocking Solution, Ready To Use, 100 ml

IW-1300 -
EUR 125

IHC-Tek Antibody Diluent pH 7.4, Ready To Use, 100 ml

IW-1000 -
EUR 125

IHC-Tek Antibody Diluent pH 8.4, Ready To Use, 100 ml

IW-1001 -
EUR 135

IHC-Tek Nuclear Fast Red Solution, Ready To Use, 250 ml

IW-1401 -
EUR 125

AutoFrost Enhanced IHC Microscope Slides, Clipped corner, White, 3000pcs/case

EMP00W3 -
EUR 1250

IHC-Tek Avidin/Biotin Blocking Solution, Ready To Use, 20ml/20ml

IW-1301 -
EUR 185

IHC-Tek Washing Buffer PBS-Tween 20, Ready To Use. 500 ml

IW-1200 -
EUR 58

IHC-Tek Washing Buffer PBS-Tween 20, Ready To Use, 1000 ml

IW-1200-1L -
EUR 85

IHC-Tek Washing Buffer TBS-Tween 20, Ready To Use, 500 ml

IW-1201 -
EUR 58

IHC-Tek Washing Buffer TBS-Tween 20, Ready To Use, 1000 ml

IW-1201-1L -
EUR 85

StainTray IHC Slide Staining System, Base with Clear Lid for 10 Slides

M918-1 -
EUR 185

StainTray IHC Slide Staining System, Base with Black Lid for 10 Slides

M918-2 -
EUR 185

StainTray IHC Slide Staining System, Base with Clear Lid for 20 Slides

M920-1 -
EUR 235

StainTray IHC Slide Staining System, Base with Black Lid for 20 Slides

M920-2 -
EUR 235

IHC-Tek HRP Anti-Goat Polymer Detection Kit - 10 ml Ready to Use

SV0003-1 -
EUR 245

IHC-Tek HRP Anti-Mouse Polymer Detection Kit - 10 ml Ready to Use

SV0001-1 -
EUR 245

IHC-Tek HRP Anti-Rabbit IgG Polymer Detection Kit - 10 ml Ready to Use

SV0002-1 -
EUR 245

IHC DAB solution

E22-NS000 120 T 
EUR 225

50ml Plug-Seal Centrifuge Tubes with Rack, 25 tubes/rack

10-0501 -
EUR 12.5

Reagent Alcohol 100%, 1 gallon

6900-1 -
EUR 78

Reagent Alcohol 80%, 1 gallon

6980-1 -
EUR 78

100x15mm Petri Dishes, 10 pcs/bag

66-1501 -
EUR 9.5

60x15mm Petri Dishes, 10 pcs/bag

66-1560 -
EUR 5.8

Bouins Fluid, 32 oz.

11750-32 -
EUR 65

3 ml Transfer Pipets, 500 pcs/pack

30-0138 -
EUR 19

10 µl Pipet Tips with Rack Sterile, 96 tips/rack

21-0010 -
EUR 6.5

200 µl Pipet Tips with Rack Sterile, 96 tips/rack

21-0200 -
EUR 6.5

1 ml Pipet Tips with Rack Sterile, 100 tips/rack

21-1000 -
EUR 12.5

Formaldehyde, 37-40%, 1 gallon

28530-1 -
EUR 85.6

Formalin, 10% Neutral Buffered, 1 gallon

28600-1 -
EUR 68

Methyl Alcohol, ACS Grade, 1 gallon

40380-1 -
EUR 78

2-place Cardboard Slide Mailer, 10 pcs/pack

41-4002 -
EUR 5.8

GB-Mount, Aqueous, 18 ml

E01-18 -
EUR 98

Simpo-Mount, Aqueous, 100 ml

E03-100 -
EUR 165

Simpo-Mount, Aqueous, 18 ml

E03-18 -
EUR 98

Fluorescent Mounting Medium with PI, 18 ml

E20-18 -
EUR 165

Fluorescent Mounting Medium with DAPI, 18 ml

E19-18 -
EUR 125

Fluorescent Mounting Medium, 18 ml

E18-18 -
EUR 98

ISH Mounting Medium, 18 ml

E21-18 -
EUR 98

Histology/Tissue Processing Cassettes - Blue, 500 pcs/pack

EC-0109B -
EUR 98
Scroll to Top