[Eukaryotic expression, protein purification and biological effects research of human CS1-Fc fusion protein].

Signaling lymphocyte activation family 7 (SLAMF7/CS1) is a cell surface glycoprotein that is highly expressed in multiple myeloma cells. CS1 is a sensitive and specific biomarker for multiple myeloma. CAR-T cell immunotherapy is a new method for the treatment of multiple myeloma. CS1 CAR-T cell immunotherapy has good effect on relapsed refractory multiple myeloma.

To detect the expression efficiency of CS1 CAR on CS1 CAR-T cells and to find an auxiliary means to CAR-T cell immunotherapy, we prepared a CS1-Fc fusion protein. First, the extracellular domain of CS1 was amplified from the existing plasmid by PCR and ligated with human IgG1-Fc fragment by overlap extension PCR.

The recombinant fragment was ligated into pMH3 eukaryotic expression vector. After restriction enzyme digestion and DNA sequencing, the pMH3-CS1-Fc-his recombinant plasmid was successfully constructed. The recombinant plasmid was transfected into Chinese hamster ovary cell (CHO-S) by liposome. The expression of the CS1-Fc fusion protein in CHO-S cells was identified by flow cytometry after G418 pressure screening. Next, the CS1-Fc fusion protein was purified by nickel column. Western-blot analysis showed that molecular weight of the fusion protein was about 70 kDa was identified by Western blotting.

The CS1-Fc fusion protein couldeffectively detect the expression rate of CS1 CAR and promote the activation, proliferation andcytokines secretion of the CS1 CAR-T cells. The results will lay the experimental foundation for the in vitro detection and potentiation of CAR-T cells in multiple myeloma treated with CS1 CAR-T cell.

Probiotics are live microorganisms that provide health benefits to the host when consumed in adequate concentrations. The strains most frequently used as probiotics include Lactobacillus and Bifidobacteria. Probiotics have demonstrated significant potential as therapeutic options for various diseases. In addition to oligosaccharides, proteins, hydrolysates and peptides have also been shown function as prebiotics to promote the growth of probiotics.

Therefore, this review provides a summary of the available information and current knowledge on the effects of various proteins on probiotics, focusing on how proteins influence probiotics, although uncertainties and disagreements about how the metabolism of proteins promotes probiotics still exist. Understanding the relationship between proteins and probiotics will allow appropriate prebiotic selection and the development of effective methods to promote the proliferation of probiotics.

DAB Chromogen Concentrate

ACB060 60 ml
EUR 165

DAB Chromogen Concentrate

ACB125 125 ml
EUR 245

DAB Chromogen Concentrate

ACB500 500 ml
EUR 671

DAB Chromogen Concentrate

ACB999 1000 ml
EUR 1159

DAB Chromogen, 4ml

NB314D 4 ml
EUR 246

DAB Chromogen/Substrate Kit

ACH500 500 Slides
EUR 85

DAB Chromogen/Substrate Bulk pack

ACK500 530 ml
EUR 120

DAB Chromogen/Substrate Bulk pack

ACK999 1060 ml
EUR 176

DAB Chromogen/Substrate Kit (High Contrast)

ACT500 500 Slides
EUR 157

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV250 265 ml
EUR 125

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV500 530 ml
EUR 165

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV999 1060 ml
EUR 236

STAT-Q Mouse Adsorbed DAB KIT; for staining Rat antibodies-on-Mouse tissues 250 plus slide kit

NB314MAK-DAB 200-250 slides
EUR 856

STAT-Q Rat Adsorbed DAB Kit: for staining Mouse antibodies-on-Rat tissues 250 plus slides kit

NB314RAK-DAB 200-250 slides
EUR 856

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml

NB319-SBB 30 ml
EUR 369

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW

NB319-BB70 70 ml
EUR 485

DAB

HY-15912 500mg
EUR 108

AEC Chromogen Kit

abx097196-1Kit 1 Kit
EUR 272

AEC Chromogen Concentrate

A3610-002 20ml
EUR 178

AEC Chromogen Concentrate

A3610-005 50ml
EUR 286

AEC Chromogen Concentrate

A3610-010 100ml
EUR 448

AEC Chromogen Concentrate

ACD015 15 ml
EUR 102

AEC Chromogen Concentrate

ACD030 30 ml
EUR 148

AEC Chromogen Concentrate

ACD125 125 ml
EUR 245

AEC Chromogen Concentrate

ACD500 500 ml
EUR 671

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04727 5g Ask for price

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04728 25g Ask for price

DAB Tablet

D3225-020 20TB
EUR 142

DAB Tablet

D3225-050 50TB
EUR 246

AEC Chromogen/Substrate Kit

A3611-500 500 slides
EUR 188

AEC Chromogen/Substrate Kit

ACG500 500 Slides
EUR 85

UltraBrite? Red IHC chromogen (AP)

M1305-30
EUR 349

UltraBrite? Blue IHC chromogen (AP)

M1307-30
EUR 370

UltraBrite? Blue IHC chromogen (HRP)

M1308-30
EUR 457

UltraBrite? Green IHC chromogen (AP)

M1309-30
EUR 425

UltraBrite? Yellow IHC chromogen (HRP)

M1310-30
EUR 457

UltraBrite? Black IHC chromogen (HRP)

M1311-30
EUR 457

UltraBrite? Red IHC chromogen (HRP)

M1312-30
EUR 370

AEC Chromogen/Substrate Bulk Kit

ACJ500 515 ml
EUR 120

AEC Chromogen/Substrate Bulk Kit

ACJ999 1030 ml
EUR 176

Fast Red Substrate/chromogen, 70ml

NB317SBF 70 ml
EUR 461

H-Dab-OH·HCl

5-04703 5g Ask for price

H-Dab-OH·HCl

5-04704 25g Ask for price

Boc-Dab-OH

5-04711 5g Ask for price

Boc-Dab-OH

5-04712 25g Ask for price

Fmoc-Dab-OAll·HCl

5-04720 5g Ask for price

Fmoc-Dab-OH·HCl

5-04721 5g Ask for price

Fmoc-Dab-OH·HCl

5-04722 25g Ask for price

Fmoc-Dab-OH

5-04735 1g Ask for price

Fmoc-Dab-OH

5-04736 5g Ask for price

DAB Substrate kit

30015 1Kit
EUR 143
Description: Minimum order quantity: 1 unit of 1Kit

Boc-Dab-OH

A-3215.0001 1.0g
EUR 126
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0005 5.0g
EUR 418
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0025 25.0g
EUR 1578
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Fmoc-Dab-OH

B-2300.0001 1.0g
EUR 151
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

Fmoc-Dab-OH

B-2300.0005 5.0g
EUR 515
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

DL-Dab.2HCl

A6727-100000 100 g
EUR 569
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-25000 25 g
EUR 309
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-5000 5 g
EUR 180
Description: DL-Dab.2HCl

DAB Substrate Buffer

ACC500 500 ml
EUR 72

DAB Substrate Buffer

ACC999 1000 ml
EUR 80

DAB Enhancer Solution

ACM030 30 ml
EUR 71

DAB Away Kit

ACP001 500 ml
EUR 98

DAB Differentiating Solution

DDS500 500 ml
EUR 109

Z-Dab-OH

C-3705.0001 1.0g
EUR 115
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0005 5.0g
EUR 273
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0025 25.0g
EUR 998
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

H-Dab-OH

F-3050.0001 1.0g
EUR 115
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0005 5.0g
EUR 225
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0025 25.0g
EUR 803
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

UltraBrite? Red IHC chromogen (AP Plus)

M1306-30
EUR 697

DAB Enhancer for ampliying DAB stains in IHC staining, 60ml

NB308 60 ml
EUR 427

DAB Enhancer for ampliying DAB stains in IHC staining, 30ml

NB308-30 30 ml
EUR 299

H-Dab(Z)-OH

5-04707 5g Ask for price

H-Dab(Z)-OH

5-04708 25g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04713 1g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04714 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04715 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04716 25g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04717 5g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04718 25g Ask for price

Fmoc-Dab(Ac)-OH

5-04723 5g Ask for price

Fmoc-Dab(Ac)-OH

5-04724 25g Ask for price

Fmoc-Dab(Boc)-OH

5-04725 5g Ask for price

Fmoc-Dab(Boc)-OH

5-04726 25g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04729 1g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04730 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04731 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04732 25g Ask for price

Fmoc-Dab(Mtt)-OH

5-04733 1g Ask for price

Fmoc-Dab(Mtt)-OH

5-04734 5g Ask for price

Fmoc-Dab(Dde)-OH

5-04739 1g Ask for price

Fmoc-Dab(Dde)-OH

5-04740 5g Ask for price

Fmoc-Dab(ivDde)-OH

5-04741 1g Ask for price

Fmoc-Dab(ivDde)-OH

5-04742 5g Ask for price

Fmoc-Dab(Z)-OH

5-04743 1g Ask for price

Fmoc-Dab(Z)-OH

5-04744 5g Ask for price

Boc-D-Dab-OH

5-04745 1g Ask for price

Boc-D-Dab-OH

5-04746 5g Ask for price

Fmoc-D-Dab-OH

5-04751 1g Ask for price

Fmoc-D-Dab-OH

5-04752 5g Ask for price

Fmoc-Dab(Fmoc)-Oh

abx188721-250g 250 g
EUR 1720

H-Dab(Boc)-OH

A-3305.0001 1.0g
EUR 200
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

H-Dab(Boc)-OH

A-3305.0005 5.0g
EUR 696
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

Boc-Dab(Fmoc)-OH

A-3520.0001 1.0g
EUR 225
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Fmoc)-OH

A-3520.0005 5.0g
EUR 780
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Aloc)-OH

A-4125.0001 1.0g
EUR 309
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-Dab(Aloc)-OH

A-4125.0005 5.0g
EUR 1143
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-D-Dab-OH

A-4215.0001 1.0g
EUR 418
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-D-Dab-OH

A-4215.0005 5.0g
EUR 1554
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-Dab-OtBu · HCl

A-4415.0001 1.0g
EUR 309
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Boc-Dab-OtBu · HCl

A-4415.0005 5.0g
EUR 1143
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Sensitive DAB Stain Kit

PW023 5Preps, 5prep
EUR 69.14

Fmoc-Dab(Boc)-OH

B-1800.0001 1.0g
EUR 200
Description: Sum Formula: C24H28N2O6; CAS# [125238-99-5]

Fmoc-Dab(Boc)-OH

B-1800.0005 5.0g
EUR 683
Description: Sum Formula: C24H28N2O6; CAS# [125238-99-5]

Fmoc-Dab(Fmoc)-OH

B-2270.0001 1.0g
EUR 236
Description: Sum Formula: C34H30N2O6; CAS# [201473-83-8]

Fmoc-Dab(Fmoc)-OH

B-2270.0005 5.0g
EUR 866
Description: Sum Formula: C34H30N2O6; CAS# [201473-83-8]

Fmoc-D-Dab-OH

B-2365.0001 1.0g
EUR 418
Description: Sum Formula: C19H20N2O4; CAS# [201484-12-0]

Fmoc-D-Dab-OH

B-2365.0005 5.0g
EUR 1554
Description: Sum Formula: C19H20N2O4; CAS# [201484-12-0]

Fmoc-Dab(Aloc)-OH

B-2850.0001 1.0g
EUR 297
Description: Sum Formula: C23H24N2O6; CAS# [204316-32-5]

Fmoc-Dab(Aloc)-OH

B-2850.0005 5.0g
EUR 1106
Description: Sum Formula: C23H24N2O6; CAS# [204316-32-5]

Fmoc-Dab(Z)-OH

B-3250.0001 1.0g
EUR 261
Description: Sum Formula: C27H26N2O6; CAS# [252049-08-4]

Fmoc-Dab(Z)-OH

B-3250.0005 5.0g
EUR 925
Description: Sum Formula: C27H26N2O6; CAS# [252049-08-4]

DAB Substrate (High Contrast)

ACU250 250 ml
EUR 85

DAB Substrate (High Contrast)

ACU500 500 ml
EUR 98

DAB Substrate (High Contrast)

ACU999 1000 ml
EUR 119

10-DAB (10-Deacetylbaccatin)

A4395-25 25 mg
EUR 514
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-5 5 mg
EUR 166
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-5.1 10 mM (in 1mL DMSO)
EUR 293
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-S Evaluation Sample
EUR 81
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

DAB (Post) Enhancing Solution

DES500 500 ml
EUR 88

DAB (Post) Enhancing Solution

DES999 1000 ml
EUR 109

Fmoc-Dab(ivDde)-OH

B-4065.0001 1.0g
EUR 297
Description: Sum Formula: C32H38N2O6; CAS# [607366-21-2]

Fmoc-Dab(ivDde)-OH

B-4065.0005 5.0g
EUR 1093
Description: Sum Formula: C32H38N2O6; CAS# [607366-21-2]

Z-Dab(Z)-OH

C-3690.0001 1.0g
EUR 200
Description: Sum Formula: C20H22N2O6; CAS# [55478-23-4]

Z-Dab(Z)-OH

C-3690.0005 5.0g
EUR 696
Description: Sum Formula: C20H22N2O6; CAS# [55478-23-4]

Z-D-Dab-OH

C-3770.0001 1.0g
EUR 225
Description: Sum Formula: C12H16N2O4; CAS# [70882-66-5]

Z-D-Dab-OH

C-3770.0005 5.0g
EUR 792
Description: Sum Formula: C12H16N2O4; CAS# [70882-66-5]

DAB Substrate buffer, 70ml

NB314SB 70 ml
EUR 276

Ac-Dab(Boc)-OH

F-1020.0250 250.0mg
EUR 236
Description: Sum Formula: C11H20N2O5; CAS# [201351-32-8]

Ac-Dab(Boc)-OH

F-1020.1000 1.0g
EUR 660
Description: Sum Formula: C11H20N2O5; CAS# [201351-32-8]

H-D-Dab-OH

F-3055.0001 1.0g
EUR 225
Description: Sum Formula: C4H10N2O2; CAS# [26908-94-1] net

H-D-Dab-OH

F-3055.0005 5.0g
EUR 792
Description: Sum Formula: C4H10N2O2; CAS# [26908-94-1] net

7,10-Dimethoxy-10-DAB III

TBZ3002 20mg Ask for price

Boc-D-Dab(Fmoc)-OH

5-04747 5g Ask for price

Boc-D-Dab(Fmoc)-OH

5-04748 25g Ask for price

Boc-D-Dab(Z)-OH·DCHA

5-04749 5g Ask for price

Boc-D-Dab(Z)-OH·DCHA

5-04750 25g Ask for price

Fmoc-D-Dab(Alloc)-OH

5-04753 1g Ask for price

Fmoc-D-Dab(Alloc)-OH

5-04754 5g Ask for price

Fmoc-D-Dab(Boc)-OH

5-04755 1g Ask for price

Fmoc-D-Dab(Boc)-OH

5-04756 5g Ask for price

Fmoc-D-Dab(Dde)-OH

5-04757 1g Ask for price

Fmoc-D-Dab(Dde)-OH

5-04758 5g Ask for price

Fmoc-D-Dab(MMt)-OH

5-04759 5g Ask for price

Fmoc-D-Dab(MMt)-OH

5-04760 25g Ask for price

Fmoc-D-Dab(Z)-OH

5-04761 250mg Ask for price

Fmoc-D-Dab(Z)-OH

5-04762 1g Ask for price

H-D-Dab-OH·2HCl

5-04763 5g Ask for price

H-D-Dab-OH·2HCl

5-04764 25g Ask for price

DAB Chromogenic Substrate Kit (Brown)

AR1022 1 kit (for 600-900 assays)
EUR 80

DAB Chromogenic Substrate Kit (Blue)

AR1025 1 kit (for 600-900 assays)
EUR 80

DAB Horseradish Peroxidase Chromogenic Kit

20-abx090660
  • EUR 230.00
  • EUR 189.00
  • 100 ml
  • 20 ml

Boc-Dab(Z)-OH · DCHA

A-2905.0001 1.0g
EUR 126
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [16947-89-0]

Boc-Dab(Z)-OH · DCHA

A-2905.0005 5.0g
EUR 393
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [16947-89-0]

Boc-Dab(Boc)-OH · DCHA

A-3480.0001 1.0g
EUR 176
Description: Sum Formula: C14H26N2O6·C12H23N; CAS# [201472-66-4]

Boc-Dab(Boc)-OH · DCHA

A-3480.0005 5.0g
EUR 587
Description: Sum Formula: C14H26N2O6·C12H23N; CAS# [201472-66-4]

Boc-D-Dab(Fmoc)-OH

A-4230.0001 1.0g
EUR 393
Description: Sum Formula: C24H28N2O6; CAS# [131570-57-5]

Boc-D-Dab(Fmoc)-OH

A-4230.0005 5.0g
EUR 1482
Description: Sum Formula: C24H28N2O6; CAS# [131570-57-5]

Fmoc-D-Dab(Boc)-OH

B-2960.0001 1.0g
EUR 393
Description: Sum Formula: C24H28N2O6; CAS# [114360-56-4]

Fmoc-D-Dab(Boc)-OH

B-2960.0005 5.0g
EUR 1482
Description: Sum Formula: C24H28N2O6; CAS# [114360-56-4]

H-D-Dab-OH.2HCl

A7493-1000 1 g
EUR 97
Description: H-D-Dab-OH?2HCl

H-D-Dab-OH.2HCl

A7493-25000 25 g
EUR 808
Description: H-D-Dab-OH?2HCl

H-D-Dab-OH.2HCl

A7493-5000 5 g
EUR 232
Description: H-D-Dab-OH?2HCl

EconoTek HRP Anti-Polyvalent (DAB)

AEX080 70 Slides
EUR 133

H-Dab(Boc)-OMe · HCl

E-3360.0001 1.0g
EUR 225
Description: Sum Formula: C10H20N2O4·HCl; CAS# [3350-15-0]

H-Dab(Boc)-OMe · HCl

E-3360.0005 5.0g
EUR 792
Description: Sum Formula: C10H20N2O4·HCl; CAS# [3350-15-0]

H-Dab(Boc)-OMe · HCl

E-3360.0025 25.0g
EUR 3078
Description: Sum Formula: C10H20N2O4·HCl; CAS# [3350-15-0]

Z-Dab(Boc)-OH · DCHA

C-3510.0001 1.0g
EUR 176
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [3350-13-8]

Z-Dab(Boc)-OH · DCHA

C-3510.0005 5.0g
EUR 587
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [3350-13-8]

H-D-Dab(Boc)-OH

F-4200.0001 1.0g
EUR 236
Description: Sum Formula: C9H18N2O4; CAS# [114360-55-3]

H-D-Dab(Boc)-OH

F-4200.0005 5.0g
EUR 853
Description: Sum Formula: C9H18N2O4; CAS# [114360-55-3]

3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs

CH027 50 Tabs
EUR 177

Boc-D-Dab(Z)-OH · DCHA

A-4260.0001 1.0g
EUR 334
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [101854-42-6]

Boc-D-Dab(Z)-OH · DCHA

A-4260.0005 5.0g
EUR 1240
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [101854-42-6]

Z-D-Dab(Boc)-OH · DCHA

C-3765.0001 1.0g
EUR 381
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [214852-61-6]

Z-D-Dab(Boc)-OH · DCHA

C-3765.0005 5.0g
EUR 1421
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [214852-61-6]

AEC Chromogen & Substrate (stable) 2-component AEC system, 70ml

NB314SCS 70 ml
EUR 450
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