[Eukaryotic expression, protein purification and biological effects research of human CS1-Fc fusion protein].

Signaling lymphocyte activation family 7 (SLAMF7/CS1) is a cell surface glycoprotein that is highly expressed in multiple myeloma cells. CS1 is a sensitive and specific biomarker for multiple myeloma. CAR-T cell immunotherapy is a new method for the treatment of multiple myeloma. CS1 CAR-T cell immunotherapy has good effect on relapsed refractory multiple myeloma.

To detect the expression efficiency of CS1 CAR on CS1 CAR-T cells and to find an auxiliary means to CAR-T cell immunotherapy, we prepared a CS1-Fc fusion protein. First, the extracellular domain of CS1 was amplified from the existing plasmid by PCR and ligated with human IgG1-Fc fragment by overlap extension PCR.

The recombinant fragment was ligated into pMH3 eukaryotic expression vector. After restriction enzyme digestion and DNA sequencing, the pMH3-CS1-Fc-his recombinant plasmid was successfully constructed. The recombinant plasmid was transfected into Chinese hamster ovary cell (CHO-S) by liposome. The expression of the CS1-Fc fusion protein in CHO-S cells was identified by flow cytometry after G418 pressure screening. Next, the CS1-Fc fusion protein was purified by nickel column. Western-blot analysis showed that molecular weight of the fusion protein was about 70 kDa was identified by Western blotting.

The CS1-Fc fusion protein couldeffectively detect the expression rate of CS1 CAR and promote the activation, proliferation andcytokines secretion of the CS1 CAR-T cells. The results will lay the experimental foundation for the in vitro detection and potentiation of CAR-T cells in multiple myeloma treated with CS1 CAR-T cell.

Probiotics are live microorganisms that provide health benefits to the host when consumed in adequate concentrations. The strains most frequently used as probiotics include Lactobacillus and Bifidobacteria. Probiotics have demonstrated significant potential as therapeutic options for various diseases. In addition to oligosaccharides, proteins, hydrolysates and peptides have also been shown function as prebiotics to promote the growth of probiotics.

Therefore, this review provides a summary of the available information and current knowledge on the effects of various proteins on probiotics, focusing on how proteins influence probiotics, although uncertainties and disagreements about how the metabolism of proteins promotes probiotics still exist. Understanding the relationship between proteins and probiotics will allow appropriate prebiotic selection and the development of effective methods to promote the proliferation of probiotics.

DAB Chromogen Concentrate

ACB060 60 ml
EUR 198

DAB Chromogen Concentrate

ACB125 125 ml
EUR 294

DAB Chromogen Concentrate

ACB500 500 ml
EUR 805.2

DAB Chromogen Concentrate

ACB999 1000 ml
EUR 1390.8

DAB Chromogen, 4ml

NB314D 4 ml
EUR 295.2

DAB Chromogen/Substrate Kit

ACH500 500 Slides
EUR 102

DAB Chromogen/Substrate Bulk pack

ACK500 530 ml
EUR 144

DAB Chromogen/Substrate Bulk pack

ACK999 1060 ml
EUR 211.2

DAB Chromogen/Substrate Kit (High Contrast)

ACT500 500 Slides
EUR 188.4

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV250 265 ml
EUR 150

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV500 530 ml
EUR 198

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV999 1060 ml
EUR 283.2

STAT-Q Mouse Adsorbed DAB KIT; for staining Rat antibodies-on-Mouse tissues 250 plus slide kit

NB314MAK-DAB 200-250 slides
EUR 1027.2

STAT-Q Rat Adsorbed DAB Kit: for staining Mouse antibodies-on-Rat tissues 250 plus slides kit

NB314RAK-DAB 200-250 slides
EUR 1027.2

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml

NB319-SBB 30 ml
EUR 442.8

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW

NB319-BB70 70 ml
EUR 582

DAB

HY-15912 500mg
EUR 129.6

AEC Chromogen Kit

abx097196-1Kit 1 Kit
EUR 326.4

AEC Chromogen Concentrate

A3610-002 20ml
EUR 213.6

AEC Chromogen Concentrate

A3610-005 50ml
EUR 343.2

AEC Chromogen Concentrate

A3610-010 100ml
EUR 537.6

AEC Chromogen Concentrate

ACD015 15 ml
EUR 122.4

AEC Chromogen Concentrate

ACD030 30 ml
EUR 177.6

AEC Chromogen Concentrate

ACD125 125 ml
EUR 294

AEC Chromogen Concentrate

ACD500 500 ml
EUR 805.2

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04727 5g Ask for price

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04728 25g Ask for price

DAB Tablet

D3225-020 20TB
EUR 170.4

DAB Tablet

D3225-050 50TB
EUR 295.2

AEC Chromogen/Substrate Kit

A3611-500 500 slides
EUR 225.6

AEC Chromogen/Substrate Kit

ACG500 500 Slides
EUR 102

AEC Chromogen/Substrate Bulk Kit

ACJ500 515 ml
EUR 144

AEC Chromogen/Substrate Bulk Kit

ACJ999 1030 ml
EUR 211.2

UltraBrite? Red IHC chromogen (AP)

M1305-30
EUR 418.8

UltraBrite? Blue IHC chromogen (AP)

M1307-30
EUR 444

UltraBrite? Blue IHC chromogen (HRP)

M1308-30
EUR 548.4

UltraBrite? Green IHC chromogen (AP)

M1309-30
EUR 510

UltraBrite? Yellow IHC chromogen (HRP)

M1310-30
EUR 548.4

UltraBrite? Black IHC chromogen (HRP)

M1311-30
EUR 548.4

UltraBrite? Red IHC chromogen (HRP)

M1312-30
EUR 444

Fast Red Substrate/chromogen, 70ml

NB317SBF 70 ml
EUR 553.2

H-Dab-OH·HCl

5-04703 5g Ask for price

H-Dab-OH·HCl

5-04704 25g Ask for price

Boc-Dab-OH

5-04711 5g Ask for price

Boc-Dab-OH

5-04712 25g Ask for price

Fmoc-Dab-OAll·HCl

5-04720 5g Ask for price

Fmoc-Dab-OH·HCl

5-04721 5g Ask for price

Fmoc-Dab-OH·HCl

5-04722 25g Ask for price

Fmoc-Dab-OH

5-04735 1g Ask for price

Fmoc-Dab-OH

5-04736 5g Ask for price

DAB Substrate kit

30015 1Kit
EUR 171.6
Description: Minimum order quantity: 1 unit of 1Kit

DL-Dab.2HCl

A6727-100000 100 g
EUR 682.8
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-25000 25 g
EUR 370.8
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-5000 5 g
EUR 216
Description: DL-Dab.2HCl

Boc-Dab-OH

A-3215.0001 1.0g
EUR 151.2
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0005 5.0g
EUR 501.6
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0025 25.0g
EUR 1893.6
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Fmoc-Dab-OH

B-2300.0001 1.0g
EUR 181.2
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

Fmoc-Dab-OH

B-2300.0005 5.0g
EUR 618
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

DAB Differentiating Solution

DDS500 500 ml
EUR 130.8

Z-Dab-OH

C-3705.0001 1.0g
EUR 138
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0005 5.0g
EUR 327.6
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0025 25.0g
EUR 1197.6
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

DAB Substrate Buffer

ACC500 500 ml
EUR 86.4

DAB Substrate Buffer

ACC999 1000 ml
EUR 96

DAB Enhancer Solution

ACM030 30 ml
EUR 85.2

DAB Away Kit

ACP001 500 ml
EUR 117.6

H-Dab-OH

F-3050.0001 1.0g
EUR 138
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0005 5.0g
EUR 270
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0025 25.0g
EUR 963.6
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

UltraBrite? Red IHC chromogen (AP Plus)

M1306-30
EUR 836.4

DAB Enhancer for ampliying DAB stains in IHC staining, 60ml

NB308 60 ml
EUR 512.4

DAB Enhancer for ampliying DAB stains in IHC staining, 30ml

NB308-30 30 ml
EUR 358.8

H-Dab(Z)-OH

5-04707 5g Ask for price

H-Dab(Z)-OH

5-04708 25g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04713 1g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04714 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04715 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04716 25g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04717 5g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04718 25g Ask for price

Fmoc-Dab(Ac)-OH

5-04723 5g Ask for price

Fmoc-Dab(Ac)-OH

5-04724 25g Ask for price

Fmoc-Dab(Boc)-OH

5-04725 5g Ask for price

Fmoc-Dab(Boc)-OH

5-04726 25g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04729 1g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04730 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04731 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04732 25g Ask for price

Fmoc-Dab(Mtt)-OH

5-04733 1g Ask for price

Fmoc-Dab(Mtt)-OH

5-04734 5g Ask for price

Fmoc-Dab(Dde)-OH

5-04739 1g Ask for price

Fmoc-Dab(Dde)-OH

5-04740 5g Ask for price

Fmoc-Dab(ivDde)-OH

5-04741 1g Ask for price

Fmoc-Dab(ivDde)-OH

5-04742 5g Ask for price

Fmoc-Dab(Z)-OH

5-04743 1g Ask for price

Fmoc-Dab(Z)-OH

5-04744 5g Ask for price

Boc-D-Dab-OH

5-04745 1g Ask for price

Boc-D-Dab-OH

5-04746 5g Ask for price

Fmoc-D-Dab-OH

5-04751 1g Ask for price

Fmoc-D-Dab-OH

5-04752 5g Ask for price

10-DAB (10-Deacetylbaccatin)

A4395-25 25 mg
EUR 616.8
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-5 5 mg
EUR 199.2
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-5.1 10 mM (in 1mL DMSO)
EUR 351.6
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-S Evaluation Sample
EUR 97.2
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

Fmoc-Dab(Fmoc)-Oh

abx188721-250g 250 g
EUR 2064

H-Dab(Boc)-OH

A-3305.0001 1.0g
EUR 240
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

H-Dab(Boc)-OH

A-3305.0005 5.0g
EUR 835.2
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

Boc-Dab(Fmoc)-OH

A-3520.0001 1.0g
EUR 270
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Fmoc)-OH

A-3520.0005 5.0g
EUR 936
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Aloc)-OH

A-4125.0001 1.0g
EUR 370.8
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-Dab(Aloc)-OH

A-4125.0005 5.0g
EUR 1371.6
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-D-Dab-OH

A-4215.0001 1.0g
EUR 501.6
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-D-Dab-OH

A-4215.0005 5.0g
EUR 1864.8
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-Dab-OtBu · HCl

A-4415.0001 1.0g
EUR 370.8
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Boc-Dab-OtBu · HCl

A-4415.0005 5.0g
EUR 1371.6
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Fmoc-Dab(Boc)-OH

B-1800.0001 1.0g
EUR 240
Description: Sum Formula: C24H28N2O6; CAS# [125238-99-5]

Fmoc-Dab(Boc)-OH

B-1800.0005 5.0g
EUR 819.6
Description: Sum Formula: C24H28N2O6; CAS# [125238-99-5]

Fmoc-Dab(Fmoc)-OH

B-2270.0001 1.0g
EUR 283.2
Description: Sum Formula: C34H30N2O6; CAS# [201473-83-8]

Fmoc-Dab(Fmoc)-OH

B-2270.0005 5.0g
EUR 1039.2
Description: Sum Formula: C34H30N2O6; CAS# [201473-83-8]

Fmoc-D-Dab-OH

B-2365.0001 1.0g
EUR 501.6
Description: Sum Formula: C19H20N2O4; CAS# [201484-12-0]

Fmoc-D-Dab-OH

B-2365.0005 5.0g
EUR 1864.8
Description: Sum Formula: C19H20N2O4; CAS# [201484-12-0]

Fmoc-Dab(Aloc)-OH

B-2850.0001 1.0g
EUR 356.4
Description: Sum Formula: C23H24N2O6; CAS# [204316-32-5]

Fmoc-Dab(Aloc)-OH

B-2850.0005 5.0g
EUR 1327.2
Description: Sum Formula: C23H24N2O6; CAS# [204316-32-5]

Fmoc-Dab(Z)-OH

B-3250.0001 1.0g
EUR 313.2
Description: Sum Formula: C27H26N2O6; CAS# [252049-08-4]

Fmoc-Dab(Z)-OH

B-3250.0005 5.0g
EUR 1110
Description: Sum Formula: C27H26N2O6; CAS# [252049-08-4]

Fmoc-Dab(ivDde)-OH

B-4065.0001 1.0g
EUR 356.4
Description: Sum Formula: C32H38N2O6; CAS# [607366-21-2]

Fmoc-Dab(ivDde)-OH

B-4065.0005 5.0g
EUR 1311.6
Description: Sum Formula: C32H38N2O6; CAS# [607366-21-2]

DAB (Post) Enhancing Solution

DES500 500 ml
EUR 105.6

DAB (Post) Enhancing Solution

DES999 1000 ml
EUR 130.8

Z-Dab(Z)-OH

C-3690.0001 1.0g
EUR 240
Description: Sum Formula: C20H22N2O6; CAS# [55478-23-4]

Z-Dab(Z)-OH

C-3690.0005 5.0g
EUR 835.2
Description: Sum Formula: C20H22N2O6; CAS# [55478-23-4]

Z-D-Dab-OH

C-3770.0001 1.0g
EUR 270
Description: Sum Formula: C12H16N2O4; CAS# [70882-66-5]

Z-D-Dab-OH

C-3770.0005 5.0g
EUR 950.4
Description: Sum Formula: C12H16N2O4; CAS# [70882-66-5]

DAB Substrate (High Contrast)

ACU250 250 ml
EUR 102

DAB Substrate (High Contrast)

ACU500 500 ml
EUR 117.6

DAB Substrate (High Contrast)

ACU999 1000 ml
EUR 142.8

DAB Substrate buffer, 70ml

NB314SB 70 ml
EUR 331.2

Ac-Dab(Boc)-OH

F-1020.0250 250.0mg
EUR 283.2
Description: Sum Formula: C11H20N2O5; CAS# [201351-32-8]

Ac-Dab(Boc)-OH

F-1020.1000 1.0g
EUR 792
Description: Sum Formula: C11H20N2O5; CAS# [201351-32-8]

H-D-Dab-OH

F-3055.0001 1.0g
EUR 270
Description: Sum Formula: C4H10N2O2; CAS# [26908-94-1] net

H-D-Dab-OH

F-3055.0005 5.0g
EUR 950.4
Description: Sum Formula: C4H10N2O2; CAS# [26908-94-1] net

Sensitive DAB Stain Kit

PW023 5Preps, 5prep
EUR 82.97

Boc-D-Dab(Fmoc)-OH

5-04747 5g Ask for price

Boc-D-Dab(Fmoc)-OH

5-04748 25g Ask for price

Boc-D-Dab(Z)-OH·DCHA

5-04749 5g Ask for price

Boc-D-Dab(Z)-OH·DCHA

5-04750 25g Ask for price

Fmoc-D-Dab(Alloc)-OH

5-04753 1g Ask for price

Fmoc-D-Dab(Alloc)-OH

5-04754 5g Ask for price

Fmoc-D-Dab(Boc)-OH

5-04755 1g Ask for price

Fmoc-D-Dab(Boc)-OH

5-04756 5g Ask for price

Fmoc-D-Dab(Dde)-OH

5-04757 1g Ask for price

Fmoc-D-Dab(Dde)-OH

5-04758 5g Ask for price

Fmoc-D-Dab(MMt)-OH

5-04759 5g Ask for price

Fmoc-D-Dab(MMt)-OH

5-04760 25g Ask for price

Fmoc-D-Dab(Z)-OH

5-04761 250mg Ask for price

Fmoc-D-Dab(Z)-OH

5-04762 1g Ask for price

H-D-Dab-OH·2HCl

5-04763 5g Ask for price

H-D-Dab-OH·2HCl

5-04764 25g Ask for price

DAB Horseradish Peroxidase Chromogenic Kit

20-abx090660
  • EUR 276.00
  • EUR 226.80
  • 100 ml
  • 20 ml

H-D-Dab-OH.2HCl

A7493-1000 1 g
EUR 116.4
Description: H-D-Dab-OH?2HCl

H-D-Dab-OH.2HCl

A7493-25000 25 g
EUR 969.6
Description: H-D-Dab-OH?2HCl

H-D-Dab-OH.2HCl

A7493-5000 5 g
EUR 278.4
Description: H-D-Dab-OH?2HCl

Boc-Dab(Z)-OH · DCHA

A-2905.0001 1.0g
EUR 151.2
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [16947-89-0]

Boc-Dab(Z)-OH · DCHA

A-2905.0005 5.0g
EUR 471.6
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [16947-89-0]

Boc-Dab(Boc)-OH · DCHA

A-3480.0001 1.0g
EUR 211.2
Description: Sum Formula: C14H26N2O6·C12H23N; CAS# [201472-66-4]

Boc-Dab(Boc)-OH · DCHA

A-3480.0005 5.0g
EUR 704.4
Description: Sum Formula: C14H26N2O6·C12H23N; CAS# [201472-66-4]

Boc-D-Dab(Fmoc)-OH

A-4230.0001 1.0g
EUR 471.6
Description: Sum Formula: C24H28N2O6; CAS# [131570-57-5]

Boc-D-Dab(Fmoc)-OH

A-4230.0005 5.0g
EUR 1778.4
Description: Sum Formula: C24H28N2O6; CAS# [131570-57-5]

Fmoc-D-Dab(Boc)-OH

B-2960.0001 1.0g
EUR 471.6
Description: Sum Formula: C24H28N2O6; CAS# [114360-56-4]

Fmoc-D-Dab(Boc)-OH

B-2960.0005 5.0g
EUR 1778.4
Description: Sum Formula: C24H28N2O6; CAS# [114360-56-4]

DAB Chromogenic Substrate Kit (Brown)

AR1022 1 kit (for 600-900 assays)
EUR 96

DAB Chromogenic Substrate Kit (Blue)

AR1025 1 kit (for 600-900 assays)
EUR 96

Z-Dab(Boc)-OH · DCHA

C-3510.0001 1.0g
EUR 211.2
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [3350-13-8]

Z-Dab(Boc)-OH · DCHA

C-3510.0005 5.0g
EUR 704.4
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [3350-13-8]

EconoTek HRP Anti-Polyvalent (DAB)

AEX080 70 Slides
EUR 159.6

H-Dab(Boc)-OMe · HCl

E-3360.0001 1.0g
EUR 270
Description: Sum Formula: C10H20N2O4·HCl; CAS# [3350-15-0]

H-Dab(Boc)-OMe · HCl

E-3360.0005 5.0g
EUR 950.4
Description: Sum Formula: C10H20N2O4·HCl; CAS# [3350-15-0]

H-Dab(Boc)-OMe · HCl

E-3360.0025 25.0g
EUR 3693.6
Description: Sum Formula: C10H20N2O4·HCl; CAS# [3350-15-0]

H-D-Dab(Boc)-OH

F-4200.0001 1.0g
EUR 283.2
Description: Sum Formula: C9H18N2O4; CAS# [114360-55-3]

H-D-Dab(Boc)-OH

F-4200.0005 5.0g
EUR 1023.6
Description: Sum Formula: C9H18N2O4; CAS# [114360-55-3]

7,10-Dimethoxy-10-DAB III

TBZ3002 20mg Ask for price

Boc-D-Dab(Z)-OH · DCHA

A-4260.0001 1.0g
EUR 400.8
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [101854-42-6]

Boc-D-Dab(Z)-OH · DCHA

A-4260.0005 5.0g
EUR 1488
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [101854-42-6]

3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs

CH027 50 Tabs
EUR 212.4

3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs

CH028 100 Tabs
EUR 279.6

Z-D-Dab(Boc)-OH · DCHA

C-3765.0001 1.0g
EUR 457.2
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [214852-61-6]

Z-D-Dab(Boc)-OH · DCHA

C-3765.0005 5.0g
EUR 1705.2
Description: Sum Formula: C17H24N2O6·C12H23N; CAS# [214852-61-6]
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