[Eukaryotic expression, protein purification and biological effects research of human CS1-Fc fusion protein].

Signaling lymphocyte activation family 7 (SLAMF7/CS1) is a cell surface glycoprotein that is highly expressed in multiple myeloma cells. CS1 is a sensitive and specific biomarker for multiple myeloma. CAR-T cell immunotherapy is a new method for the treatment of multiple myeloma. CS1 CAR-T cell immunotherapy has good effect on relapsed refractory multiple myeloma.

To detect the expression efficiency of CS1 CAR on CS1 CAR-T cells and to find an auxiliary means to CAR-T cell immunotherapy, we prepared a CS1-Fc fusion protein. First, the extracellular domain of CS1 was amplified from the existing plasmid by PCR and ligated with human IgG1-Fc fragment by overlap extension PCR.

The recombinant fragment was ligated into pMH3 eukaryotic expression vector. After restriction enzyme digestion and DNA sequencing, the pMH3-CS1-Fc-his recombinant plasmid was successfully constructed. The recombinant plasmid was transfected into Chinese hamster ovary cell (CHO-S) by liposome. The expression of the CS1-Fc fusion protein in CHO-S cells was identified by flow cytometry after G418 pressure screening. Next, the CS1-Fc fusion protein was purified by nickel column. Western-blot analysis showed that molecular weight of the fusion protein was about 70 kDa was identified by Western blotting.

The CS1-Fc fusion protein couldeffectively detect the expression rate of CS1 CAR and promote the activation, proliferation andcytokines secretion of the CS1 CAR-T cells. The results will lay the experimental foundation for the in vitro detection and potentiation of CAR-T cells in multiple myeloma treated with CS1 CAR-T cell.

Probiotics are live microorganisms that provide health benefits to the host when consumed in adequate concentrations. The strains most frequently used as probiotics include Lactobacillus and Bifidobacteria. Probiotics have demonstrated significant potential as therapeutic options for various diseases. In addition to oligosaccharides, proteins, hydrolysates and peptides have also been shown function as prebiotics to promote the growth of probiotics.

Therefore, this review provides a summary of the available information and current knowledge on the effects of various proteins on probiotics, focusing on how proteins influence probiotics, although uncertainties and disagreements about how the metabolism of proteins promotes probiotics still exist. Understanding the relationship between proteins and probiotics will allow appropriate prebiotic selection and the development of effective methods to promote the proliferation of probiotics.

DAB Chromogen Concentrate

ACB030 30 ml
EUR 119

DAB Chromogen Concentrate

ACB060 60 ml
EUR 165

DAB Chromogen Concentrate

ACB125 125 ml
EUR 245

DAB Chromogen Concentrate

ACB500 500 ml
EUR 671

DAB Chromogen Concentrate

ACB999 1000 ml
EUR 1159

DAB Chromogen/Substrate Kit

ACH500 500 Slides
EUR 85

DAB Chromogen/Substrate Bulk pack

ACK500 530 ml
EUR 120

DAB Chromogen/Substrate Bulk pack

ACK999 1060 ml
EUR 176

DAB Chromogen/Substrate Kit (High Contrast)

ACT500 500 Slides
EUR 157

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV250 265 ml
EUR 125

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV500 530 ml
EUR 165

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV999 1060 ml
EUR 236

STAT-Q Mouse Adsorbed DAB KIT; for staining Rat antibodies-on-Mouse tissues 250 plus slide kit

NB314MAK-DAB 200-250 slides
EUR 856

STAT-Q Rat Adsorbed DAB Kit: for staining Mouse antibodies-on-Rat tissues 250 plus slides kit

NB314RAK-DAB 200-250 slides
EUR 856

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml

NB319-SBB 30 ml
EUR 369

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW

NB319-BB70 70 ml
EUR 485

DAB

HY-15912 500mg
EUR 108

AEC Chromogen Kit

abx097196-1Kit 1 Kit
EUR 272

AEC Chromogen Concentrate

A3610-002 20ml
EUR 178

AEC Chromogen Concentrate

A3610-005 50ml
EUR 286

AEC Chromogen Concentrate

A3610-010 100ml
EUR 448

AEC Chromogen Concentrate

ACD015 15 ml
EUR 102

AEC Chromogen Concentrate

ACD030 30 ml
EUR 148

AEC Chromogen Concentrate

ACD125 125 ml
EUR 245

AEC Chromogen Concentrate

ACD500 500 ml
EUR 671

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04727 5g Ask for price

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04728 25g Ask for price

DAB Tablet

D3225-020 20TB
EUR 142

DAB Tablet

D3225-050 50TB
EUR 246

AEC Chromogen/Substrate Kit

A3611-500 500 slides
EUR 188

AEC Chromogen/Substrate Kit

ACG500 500 Slides
EUR 85

Fast Red Substrate/chromogen, 70ml

NB317SBF 70 ml
EUR 461

AEC Chromogen/Substrate Bulk Kit

ACJ500 515 ml
EUR 120

AEC Chromogen/Substrate Bulk Kit

ACJ999 1030 ml
EUR 176

UltraBrite? Red IHC chromogen (AP)

M1305-30
EUR 349

UltraBrite? Blue IHC chromogen (AP)

M1307-30
EUR 370

UltraBrite? Blue IHC chromogen (HRP)

M1308-30
EUR 457

UltraBrite? Green IHC chromogen (AP)

M1309-30
EUR 425

UltraBrite? Yellow IHC chromogen (HRP)

M1310-30
EUR 457

UltraBrite? Black IHC chromogen (HRP)

M1311-30
EUR 457

UltraBrite? Red IHC chromogen (HRP)

M1312-30
EUR 370

H-Dab-OH·HCl

5-04703 5g Ask for price

H-Dab-OH·HCl

5-04704 25g Ask for price

Boc-Dab-OH

5-04711 5g Ask for price

Boc-Dab-OH

5-04712 25g Ask for price

Fmoc-Dab-OAll·HCl

5-04720 5g Ask for price

Fmoc-Dab-OH·HCl

5-04721 5g Ask for price

Fmoc-Dab-OH·HCl

5-04722 25g Ask for price

Fmoc-Dab-OH

5-04735 1g Ask for price

Fmoc-Dab-OH

5-04736 5g Ask for price

Boc-Dab-OH

A-3215.0001 1.0g
EUR 126
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0005 5.0g
EUR 418
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0025 25.0g
EUR 1578
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

DAB Substrate kit

30015 1Kit
EUR 143
Description: Minimum order quantity: 1 unit of 1Kit

Z-Dab-OH

C-3705.0001 1.0g
EUR 115
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0005 5.0g
EUR 273
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0025 25.0g
EUR 998
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

DAB Differentiating Solution

DDS500 500 ml
EUR 109

H-Dab-OH

F-3050.0001 1.0g
EUR 115
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0005 5.0g
EUR 225
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0025 25.0g
EUR 803
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

DL-Dab.2HCl

A6727-100000 100 g
EUR 569
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-25000 25 g
EUR 309
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-5000 5 g
EUR 180
Description: DL-Dab.2HCl

DAB Substrate Buffer

ACC500 500 ml
EUR 72

DAB Substrate Buffer

ACC999 1000 ml
EUR 80

DAB Enhancer Solution

ACM030 30 ml
EUR 71

DAB Away Kit

ACP001 500 ml
EUR 98

Fmoc-Dab-OH

B-2300.0001 1.0g
EUR 151
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

Fmoc-Dab-OH

B-2300.0005 5.0g
EUR 515
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

UltraBrite? Red IHC chromogen (AP Plus)

M1306-30
EUR 697

DAB Enhancer for ampliying DAB stains in IHC staining, 60ml

NB308 60 ml
EUR 427

DAB Enhancer for ampliying DAB stains in IHC staining, 30ml

NB308-30 30 ml
EUR 299

H-Dab(Z)-OH

5-04707 5g Ask for price

H-Dab(Z)-OH

5-04708 25g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04713 1g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04714 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04715 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04716 25g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04717 5g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04718 25g Ask for price

Fmoc-Dab(Ac)-OH

5-04723 5g Ask for price

Fmoc-Dab(Ac)-OH

5-04724 25g Ask for price

Fmoc-Dab(Boc)-OH

5-04725 5g Ask for price

Fmoc-Dab(Boc)-OH

5-04726 25g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04729 1g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04730 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04731 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04732 25g Ask for price

Fmoc-Dab(Mtt)-OH

5-04733 1g Ask for price

Fmoc-Dab(Mtt)-OH

5-04734 5g Ask for price

Fmoc-Dab(Dde)-OH

5-04739 1g Ask for price

Fmoc-Dab(Dde)-OH

5-04740 5g Ask for price

Fmoc-Dab(ivDde)-OH

5-04741 1g Ask for price

Fmoc-Dab(ivDde)-OH

5-04742 5g Ask for price

Fmoc-Dab(Z)-OH

5-04743 1g Ask for price
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