Signaling lymphocyte activation family 7 (SLAMF7/CS1) is a cell surface glycoprotein that is highly expressed in multiple myeloma cells. CS1 is a sensitive and specific biomarker for multiple myeloma. CAR-T cell immunotherapy is a new method for the treatment of multiple myeloma. CS1 CAR-T cell immunotherapy has good effect on relapsed refractory multiple myeloma.
To detect the expression efficiency of CS1 CAR on CS1 CAR-T cells and to find an auxiliary means to CAR-T cell immunotherapy, we prepared a CS1-Fc fusion protein. First, the extracellular domain of CS1 was amplified from the existing plasmid by PCR and ligated with human IgG1-Fc fragment by overlap extension PCR.
The recombinant fragment was ligated into pMH3 eukaryotic expression vector. After restriction enzyme digestion and DNA sequencing, the pMH3-CS1-Fc-his recombinant plasmid was successfully constructed. The recombinant plasmid was transfected into Chinese hamster ovary cell (CHO-S) by liposome. The expression of the CS1-Fc fusion protein in CHO-S cells was identified by flow cytometry after G418 pressure screening. Next, the CS1-Fc fusion protein was purified by nickel column. Western-blot analysis showed that molecular weight of the fusion protein was about 70 kDa was identified by Western blotting.
The CS1-Fc fusion protein couldeffectively detect the expression rate of CS1 CAR and promote the activation, proliferation andcytokines secretion of the CS1 CAR-T cells. The results will lay the experimental foundation for the in vitro detection and potentiation of CAR-T cells in multiple myeloma treated with CS1 CAR-T cell.
Probiotics are live microorganisms that provide health benefits to the host when consumed in adequate concentrations. The strains most frequently used as probiotics include Lactobacillus and Bifidobacteria. Probiotics have demonstrated significant potential as therapeutic options for various diseases. In addition to oligosaccharides, proteins, hydrolysates and peptides have also been shown function as prebiotics to promote the growth of probiotics.
Therefore, this review provides a summary of the available information and current knowledge on the effects of various proteins on probiotics, focusing on how proteins influence probiotics, although uncertainties and disagreements about how the metabolism of proteins promotes probiotics still exist. Understanding the relationship between proteins and probiotics will allow appropriate prebiotic selection and the development of effective methods to promote the proliferation of probiotics.
DAB Chromogen Concentrate |
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ACB030 | ScyTek Laboratories | 30 ml | EUR 119 |
DAB Chromogen Concentrate |
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ACB060 | ScyTek Laboratories | 60 ml | EUR 165 |
DAB Chromogen Concentrate |
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ACB125 | ScyTek Laboratories | 125 ml | EUR 245 |
DAB Chromogen Concentrate |
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ACB500 | ScyTek Laboratories | 500 ml | EUR 671 |
DAB Chromogen Concentrate |
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ACB999 | ScyTek Laboratories | 1000 ml | EUR 1159 |
DAB Chromogen/Substrate Kit |
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ACH500 | ScyTek Laboratories | 500 Slides | EUR 85 |
DAB Chromogen/Substrate Bulk pack |
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ACK500 | ScyTek Laboratories | 530 ml | EUR 120 |
DAB Chromogen/Substrate Bulk pack |
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ACK999 | ScyTek Laboratories | 1060 ml | EUR 176 |
DAB Chromogen/Substrate Kit (High Contrast) |
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ACT500 | ScyTek Laboratories | 500 Slides | EUR 157 |
DAB Chromogen/Substrate Bulk Pack (High Contrast) |
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ACV250 | ScyTek Laboratories | 265 ml | EUR 125 |
DAB Chromogen/Substrate Bulk Pack (High Contrast) |
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ACV500 | ScyTek Laboratories | 530 ml | EUR 165 |
DAB Chromogen/Substrate Bulk Pack (High Contrast) |
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ACV999 | ScyTek Laboratories | 1060 ml | EUR 236 |
STAT-Q Mouse Adsorbed DAB KIT; for staining Rat antibodies-on-Mouse tissues 250 plus slide kit |
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NB314MAK-DAB | Innovex | 200-250 slides | EUR 856 |
STAT-Q Rat Adsorbed DAB Kit: for staining Mouse antibodies-on-Rat tissues 250 plus slides kit |
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NB314RAK-DAB | Innovex | 200-250 slides | EUR 856 |
Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml |
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NB319-SBB | Innovex | 30 ml | EUR 369 |
Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW |
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NB319-BB70 | Innovex | 70 ml | EUR 485 |
DAB |
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HY-15912 | MedChemExpress | 500mg | EUR 108 |
AEC Chromogen Kit |
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abx097196-1Kit | Abbexa | 1 Kit | EUR 272 |
AEC Chromogen Concentrate |
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A3610-002 | GenDepot | 20ml | EUR 178 |
AEC Chromogen Concentrate |
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A3610-005 | GenDepot | 50ml | EUR 286 |
AEC Chromogen Concentrate |
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A3610-010 | GenDepot | 100ml | EUR 448 |
AEC Chromogen Concentrate |
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ACD015 | ScyTek Laboratories | 15 ml | EUR 102 |
AEC Chromogen Concentrate |
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ACD030 | ScyTek Laboratories | 30 ml | EUR 148 |
AEC Chromogen Concentrate |
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ACD125 | ScyTek Laboratories | 125 ml | EUR 245 |
AEC Chromogen Concentrate |
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ACD500 | ScyTek Laboratories | 500 ml | EUR 671 |
Fmoc-Dab[Fmoc-Dab(Boc)]-OH |
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5-04727 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab[Fmoc-Dab(Boc)]-OH |
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5-04728 | CHI Scientific | 25g | Ask for price |
DAB Tablet |
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D3225-020 | GenDepot | 20TB | EUR 142 |
DAB Tablet |
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D3225-050 | GenDepot | 50TB | EUR 246 |
AEC Chromogen/Substrate Kit |
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A3611-500 | GenDepot | 500 slides | EUR 188 |
AEC Chromogen/Substrate Kit |
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ACG500 | ScyTek Laboratories | 500 Slides | EUR 85 |
Fast Red Substrate/chromogen, 70ml |
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NB317SBF | Innovex | 70 ml | EUR 461 |
AEC Chromogen/Substrate Bulk Kit |
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ACJ500 | ScyTek Laboratories | 515 ml | EUR 120 |
AEC Chromogen/Substrate Bulk Kit |
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ACJ999 | ScyTek Laboratories | 1030 ml | EUR 176 |
UltraBrite? Red IHC chromogen (AP) |
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M1305-30 | Biovision | EUR 349 |
UltraBrite? Blue IHC chromogen (AP) |
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M1307-30 | Biovision | EUR 370 |
UltraBrite? Blue IHC chromogen (HRP) |
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M1308-30 | Biovision | EUR 457 |
UltraBrite? Green IHC chromogen (AP) |
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M1309-30 | Biovision | EUR 425 |
UltraBrite? Yellow IHC chromogen (HRP) |
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M1310-30 | Biovision | EUR 457 |
UltraBrite? Black IHC chromogen (HRP) |
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M1311-30 | Biovision | EUR 457 |
UltraBrite? Red IHC chromogen (HRP) |
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M1312-30 | Biovision | EUR 370 |
H-Dab-OH·HCl |
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5-04703 | CHI Scientific | 5g | Ask for price |
H-Dab-OH·HCl |
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5-04704 | CHI Scientific | 25g | Ask for price |
Boc-Dab-OH |
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5-04711 | CHI Scientific | 5g | Ask for price |
Boc-Dab-OH |
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5-04712 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab-OAll·HCl |
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5-04720 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab-OH·HCl |
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5-04721 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab-OH·HCl |
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5-04722 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab-OH |
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5-04735 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab-OH |
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5-04736 | CHI Scientific | 5g | Ask for price |
Boc-Dab-OH |
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A-3215.0001 | Bachem | 1.0g | EUR 126 |
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6] |
Boc-Dab-OH |
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A-3215.0005 | Bachem | 5.0g | EUR 418 |
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6] |
Boc-Dab-OH |
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A-3215.0025 | Bachem | 25.0g | EUR 1578 |
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6] |
DAB Substrate kit |
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30015 | Biotium | 1Kit | EUR 143 |
Description: Minimum order quantity: 1 unit of 1Kit |
Z-Dab-OH |
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C-3705.0001 | Bachem | 1.0g | EUR 115 |
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6] |
Z-Dab-OH |
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C-3705.0005 | Bachem | 5.0g | EUR 273 |
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6] |
Z-Dab-OH |
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C-3705.0025 | Bachem | 25.0g | EUR 998 |
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6] |
DAB Differentiating Solution |
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DDS500 | ScyTek Laboratories | 500 ml | EUR 109 |
H-Dab-OH |
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F-3050.0001 | Bachem | 1.0g | EUR 115 |
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6] |
H-Dab-OH |
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F-3050.0005 | Bachem | 5.0g | EUR 225 |
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6] |
H-Dab-OH |
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F-3050.0025 | Bachem | 25.0g | EUR 803 |
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6] |
DL-Dab.2HCl |
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A6727-100000 | ApexBio | 100 g | EUR 569 |
Description: DL-Dab.2HCl |
DL-Dab.2HCl |
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A6727-25000 | ApexBio | 25 g | EUR 309 |
Description: DL-Dab.2HCl |
DL-Dab.2HCl |
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A6727-5000 | ApexBio | 5 g | EUR 180 |
Description: DL-Dab.2HCl |
DAB Substrate Buffer |
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ACC500 | ScyTek Laboratories | 500 ml | EUR 72 |
DAB Substrate Buffer |
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ACC999 | ScyTek Laboratories | 1000 ml | EUR 80 |
DAB Enhancer Solution |
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ACM030 | ScyTek Laboratories | 30 ml | EUR 71 |
DAB Away Kit |
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ACP001 | ScyTek Laboratories | 500 ml | EUR 98 |
Fmoc-Dab-OH |
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B-2300.0001 | Bachem | 1.0g | EUR 151 |
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7] |
Fmoc-Dab-OH |
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B-2300.0005 | Bachem | 5.0g | EUR 515 |
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7] |
UltraBrite? Red IHC chromogen (AP Plus) |
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M1306-30 | Biovision | EUR 697 |
DAB Enhancer for ampliying DAB stains in IHC staining, 60ml |
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NB308 | Innovex | 60 ml | EUR 427 |
DAB Enhancer for ampliying DAB stains in IHC staining, 30ml |
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NB308-30 | Innovex | 30 ml | EUR 299 |
H-Dab(Z)-OH |
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5-04707 | CHI Scientific | 5g | Ask for price |
H-Dab(Z)-OH |
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5-04708 | CHI Scientific | 25g | Ask for price |
Boc-Dab(Boc)-OH·DCHA |
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5-04713 | CHI Scientific | 1g | Ask for price |
Boc-Dab(Boc)-OH·DCHA |
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5-04714 | CHI Scientific | 5g | Ask for price |
Boc-Dab(Fmoc)-OH |
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5-04715 | CHI Scientific | 5g | Ask for price |
Boc-Dab(Fmoc)-OH |
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5-04716 | CHI Scientific | 25g | Ask for price |
Boc-Dab(Z)-OH·DCHA |
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5-04717 | CHI Scientific | 5g | Ask for price |
Boc-Dab(Z)-OH·DCHA |
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5-04718 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab(Ac)-OH |
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5-04723 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(Ac)-OH |
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5-04724 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab(Boc)-OH |
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5-04725 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(Boc)-OH |
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5-04726 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab(Fmoc)-OH |
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5-04729 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(Fmoc)-OH |
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5-04730 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(MMt)-OH |
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5-04731 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(MMt)-OH |
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5-04732 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab(Mtt)-OH |
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5-04733 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(Mtt)-OH |
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5-04734 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(Dde)-OH |
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5-04739 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(Dde)-OH |
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5-04740 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(ivDde)-OH |
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5-04741 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(ivDde)-OH |
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5-04742 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(Z)-OH |
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5-04743 | CHI Scientific | 1g | Ask for price |