E.Coli recombinant proteins, Polyclonal antibodies, Monoclonal clones and Elisa kits
A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.
The identification of circulating biomarkers that closely correlate with Parkinson’s Disease (PD) has failed several times in the past. Nevertheless, in this pilot study, a translational approach was conducted, allowing the evaluation of the plasma levels of two mitochondrial-related proteins, whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients from matched controls.
The proposed translational approach was initiated by the analysis of secretomes from cells cultured under control or well-defined oxidative stress conditions, followed by the identification of proteins related to PD pathologic mechanisms that were altered between the two states. This pipeline was further translated into the analysis of undepleted plasma samples from 28 control and 31 PD patients.From the secretome analysis, several mitochondria-related proteins were found to be differentially released between control and stress conditions and to be able to distinguish the two secretomes.
Similarly, two mitochondrial-related proteins were found to be significantly changed in a PD cohort compared to matched controls. Moreover, a linear discriminant model with potential diagnostic value to discriminate PD patients was obtained using the combination of these two proteins. Both proteins are associated with apoptotic mitochondrial changes, which may correspond to potential indicators of cell death. Moreover, one of these proteins, the VPS35 protein, was reported in plasma for the first time, and its quantification was only possible due to its previous identification in the secretome analysis.
A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.
In this work, an adaptation of a translational pipeline for biomarker selection was presented and transposed to neurological diseases, in the present case Parkinson’s Disease. The novelty and success of this pilot study may arise from the combination of: i) a translational research pipeline, where plasma samples are interrogated using knowledge previously obtained from the evaluation of cells’ secretome under oxidative stress; ii) the combined used of statistical analysis and an informed selection of candidates based on their link with relevant disease mechanisms, and iii) the use of SWATH-MS, an untargeted MS method that allows a complete record of the analyzed samples and a targeted data extraction of the quantitative values of proteins previously identified.
DAB Buffer, 10X For use with DAB Staining System for HRPO
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.
Description: Lectin Gold Staining Kit.
Kit Contents: 1mg pure Limax flavus (LFA), 5mg each of Canavalia ensiformis (Con A) and Triticum vulgare (WGA), 1ml each of Horseradish Peroxidase, Ovomucoid, Fetuin, Arachis hypogaea (PNA), Dolichos biflorus (DBA), and Ulex europaeus (UEA-I), all labeled with 15nm colloidal gold, 10ml each of the required inhibitory carbohydrates for each lectin, appropriate buffers, and 1 Silver Enhancement Kit.
Product volume may vary.
With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
