A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkers.

A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.

The identification of circulating biomarkers that closely correlate with Parkinson’s Disease (PD) has failed several times in the past. Nevertheless, in this pilot study, a translational approach was conducted, allowing the evaluation of the plasma levels of two mitochondrial-related proteins, whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients from matched controls.

The proposed translational approach was initiated by the analysis of secretomes from cells cultured under control or well-defined oxidative stress conditions, followed by the identification of proteins related to PD pathologic mechanisms that were altered between the two states. This pipeline was further translated into the analysis of undepleted plasma samples from 28 control and 31 PD patients.From the secretome analysis, several mitochondria-related proteins were found to be differentially released between control and stress conditions and to be able to distinguish the two secretomes.

Similarly, two mitochondrial-related proteins were found to be significantly changed in a PD cohort compared to matched controls. Moreover, a linear discriminant model with potential diagnostic value to discriminate PD patients was obtained using the combination of these two proteins. Both proteins are associated with apoptotic mitochondrial changes, which may correspond to potential indicators of cell death. Moreover, one of these proteins, the VPS35 protein, was reported in plasma for the first time, and its quantification was only possible due to its previous identification in the secretome analysis.

A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkers.
A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.

In this work, an adaptation of a translational pipeline for biomarker selection was presented and transposed to neurological diseases, in the present case Parkinson’s Disease. The novelty and success of this pilot study may arise from the combination of: i) a translational research pipeline, where plasma samples are interrogated using knowledge previously obtained from the evaluation of cells’ secretome under oxidative stress; ii) the combined used of statistical analysis and an informed selection of candidates based on their link with relevant disease mechanisms, and iii) the use of SWATH-MS, an untargeted MS method that allows a complete record of the analyzed samples and a targeted data extraction of the quantitative values of proteins previously identified.

UltraTek HRP Anti-Rabbit (DAB) Staining System

AKC080 70 Slides
EUR 61.72

SensiTek HRP Anti-Polyvalent (DAB) Staining System

AEM080 70 Slides
EUR 61.72

UltraTek HRP Anti-Polyvalent (DAB) Staining System

AMF080 70 Slides
EUR 70.71

STAT-Q Multivalent DAB KIT for staining Mouse, Rat

NB314KLD 1000 slides
EUR 895
Description: STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 1000 plus slides (Large size kit)

STAT-Q Multivalent DAB KIT for staining Mouse, Rat

NB314KLD-20 300 slide kit
EUR 595
Description: STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 350 slides kit (Small size Kit)

STAT-Q Goat DAB KIT for staining all Goat primary

NB314KLGD-20 20 ml kit
EUR 589
Description: STAT-Q Goat DAB KIT for staining all Goat primary antibodies, 350 plus slides

STAT-Q Rabbit DAB KIT for staining all Rabbit anti

NB314KLRD-20 20 ml kit
EUR 585
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STAT-Q Mouse Adsorbed DAB KIT for staining Rat ant

NB314MAK-DAB 200-250 slides
EUR 695
Description: STAT-Q Mouse Adsorbed DAB KIT for staining Rat antibodies-on-Mouse tissues 250 plus slide kit

STAT-Q Rat Adsorbed DAB KIT for staining Mouse ant

NB314RAK-DAB 200-250 slides
EUR 695
Description: STAT-Q Rat Adsorbed DAB KIT for staining Mouse antibodies-on-Rat tissues 250 plus slides kit

STAT Double Staining ragent for Double staining Mo

NB330DB 25 ml
EUR 595
Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides

STAT Double Staining reagent for Double staining o

NB326DB 25 ml
EUR 595
Description: STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides

STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

HE Staining Kit

E-IR-R117-100mL 100mL
EUR 240

HE Staining Kit

E-IR-R117-10mL 10mL
EUR 35

HE Staining Kit

E-IR-R117-50mL 50mL
EUR 150

HE Staining Kit

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DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

TRAP Staining Kit

KT-008 1 kit
EUR 747

DAPI Staining Kit

abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit

abx097206-1ml 1 ml Ask for price

DAPI Staining Kit

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Coplin Staining Jar

08415-3 3sets
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Silver Staining Kit

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EUR 201.16
Description: Silver Staining Kit

Cell Staining Buffer

E-CK-A107-100mL 100 mL
EUR 20

Cell Staining Buffer

E-CK-A107-200mL 200 mL
EUR 35

Cell Staining Buffer

E-CK-A107-500mL 500 mL
EUR 60

Cell Staining Buffer

E16FXP005 500 ml
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Description: Available in various conjugation types.

DNA Staining Reagent

abx299713-100g 100 µg Ask for price

DNA Staining Reagent

abx299713-20g 20 µg
EUR 300

DNA Staining Reagent

abx299713-50g 50 µg Ask for price

Cell Staining Buffer

E-CK-A107-each each Ask for price

ELVIS ID Staining Kit

SK-ELVIS-100 100 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-1000 1000 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-200 200 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-500 500 Tests Ask for price

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Hoechst 33342 Staining Kit

abx097207-100l 100 µl
EUR 162.5

Hoechst 33342 Staining Kit

abx097207-1ml 1 ml Ask for price

Hoechst 33342 Staining Kit

abx097207-200l 200 µl Ask for price

DNA STAINING AGENT (1ML)

NOVELJUICE each
EUR 80

Cell Cycle Staining Kit

KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-50T 50 T
EUR 69
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-each each Ask for price

Hematoxylin Staining Buffer

E-IR-R120-15mL 15mL
EUR 60

Hematoxylin Staining Buffer

E-IR-R120-50mL 50mL
EUR 170

Hematoxylin Staining Buffer

E-IR-R120-5mL 5mL
EUR 25

Hematoxylin Staining Buffer

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Live NucRed staining kit

EGY069 >100T
EUR 210

PI / RNase Staining Buffer

20-abx090619
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PI / RNase Staining Buffer

abx090619-100l 100 µl
EUR 162.5

PI / RNase Staining Buffer

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PI / RNase Staining Buffer

abx090619-200l 200 µl
EUR 200

HISTOCHEMISTRY STAINING TRAY

ATC-12 12 PLACE - CLEAR LID
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HISTOCHEMISTRY STAINING TRAY

ATC-24 24 PLACE - CLEAR LID
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HISTOCHEMISTRY STAINING TRAY

AT-12 12 PLACE - BLACK LID
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HISTOCHEMISTRY STAINING TRAY

AT-24 24 PLACE - BLACK LID
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Exosome fluorescent staining

S104 -
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Stains All Gel Staining Kit

KT-792 20 tests
EUR 737

Live NucGreen staining kit

EGY068 >100T
EUR 210

Dead NucGreen staining kit

EGY070 >100T
EUR 210

GEL STAINING/INCUBATION BOX

SB-10 LIGHT SHIELDING
EUR 94.44

Ponceau S Staining Solution

41640009-1 500 mL
EUR 40.13

Ponceau S Staining Solution

41640009-2 1 L
EUR 58.16

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Ponceau S Staining Solution

B7778-100000 100 g
EUR 78
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

B7778-50000 50 g
EUR 53
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Ponceau S Staining Solution

EZWB08-1x-100mL 100mL
EUR 9.6

Ponceau S Staining Solution

EZWB08-1x-500mL 500mL
EUR 32.4

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
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Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 500
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

PI Viability Staining Solution

E16FXP002 150 tests
EUR 40
Description: Available in various conjugation types.

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 390.96

β-Galactosidase Staining Kit

AKR-100 1 kit
EUR 345

Methyl Green Staining Solution

abx090698-100l 100 µl
EUR 125

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Methyl Green Staining Solution

abx090698-200l 200 µl
EUR 187.5

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 203

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Live-Dead Cell Staining Kit

GWB-AXR262 100 stainings Ask for price

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

Live-Dead Cell Staining Kit

K2081-1000 1000T
EUR 112

Live-Dead Cell Staining Kit

K2081-500 500T
EUR 68

EZ-Quick Slide Staining Set

IW-2510 -
EUR 425

Nerve Terminal Staining Kit I

70030-1 KT
EUR 605

Nerve Terminal Staining Kit V

70034 1SET
EUR 605
Description: N/A

Nerve Terminal Staining Kit V

70034-1 KT
EUR 605

Purple-Color AP Staining Kit

AP100P-1 100 Assays
EUR 235

Human Histochemistry Staining Kit

20-abx299039
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Beta-Galactosidase Staining Kit

GWB-AXR372 200 assays. Ask for price

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 274

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Slides Staining Jar, Schifferdecker

CG146-5X1NO 1 unit
EUR 53.59
Description: Slides Staining Jar, Schifferdecker

Lipid (Oil Red O) staining Kit

K580-24 each
EUR 542.4

Nerve Terminal Staining Kit IIA

70031-1 1SET
EUR 322
Description: N/A

Nerve Terminal Staining Kit IIB

70031-1-1 KT
EUR 395

Nerve Terminal Staining Kit III

70032-1 KT
EUR 513

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 418
Description: Detects activated Caspases by FACS, FL or plate reader

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 246.09

Ponceau S Staining Solution, 10×

EZWB08-10x-10mL 10mL
EUR 9

Ponceau S Staining Solution, 10×

EZWB08-10x-50mL 50mL
EUR 28.8

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Prime Juice DNA Staining Reagent

LD011-1000 1 ml
EUR 10

Novel Green DNA Staining Reagent

abx299721-100g 100 µg Ask for price

Novel Green DNA Staining Reagent

abx299721-20g 20 µg
EUR 325

Novel Green DNA Staining Reagent

abx299721-50g 50 µg Ask for price

Propidium Iodide (PI) Staining Kit

abx097208-100l 100 µl
EUR 162.5

Propidium Iodide (PI) Staining Kit

abx097208-1ml 1 ml Ask for price

Propidium Iodide (PI) Staining Kit

abx097208-200l 200 µl Ask for price

DAPI staining kit (ready to use) 

EGY0621 10mL
EUR 85

DAPI staining kit (ready to use) 

EGY0622 50mL
EUR 185

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 127.28

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 63

7-AAD Viability Staining Buffer

abx090617-100tests 100 tests
EUR 260.4

Plastic Coplin Staining Jars, each

IW-2501 -
EUR 8.6

7-AAD Viability Staining Buffer

abx090617-100l 100 µl
EUR 150

7-AAD Viability Staining Buffer

abx090617-1ml 1 ml Ask for price

7-AAD Viability Staining Buffer

abx090617-200l 200 µl Ask for price

Stayrightâ„¢ Purple HRP Staining Kit

45905 5 mL
EUR 112
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45905-5mL 5 mL
EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906 50 mL
EUR 341
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906-50mL 50 mL
EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Hematoxylin and Eosin Staining Kit

K1142-1000 2×500mL
EUR 62
Description: Hematoxylin and Eosin Staining,one of the most widely used staining technique in histopathology

TRAP Staining Kit with Osteoplates

KT-651 1 kit
EUR 1003

Hochst33342/PI Double Staining Kit

E37F032-100-tests 100tests
EUR 66.67
Description: Double staining kit

PAP PEN FOR IMMUNO STAINING - EACH

Z377821-1EA EACH
EUR 192.41

7-AAD Viability Staining Solution

E16FXP003 150 tests
EUR 145
Description: Available in various conjugation types.

7-AAD Viability Staining Solution

EXB0026 400 tests
EUR 44

Nalgene Box Staining 13x13x5cm - EACH

BOX1040 EACH
EUR 54

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 157.2

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 225.6

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 336

Disposable Slide Staining Trays - PK4

MIC5070 PK4
EUR 101.25

Slides Staining Jar, Hellendhal Type

CG145-5X1NO 1 unit
EUR 61.59
Description: Slides Staining Jar, Hellendhal Type

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 292.8

Wheaton staining dishes 3 EA - PK3

S5017-3EA PK3
EUR 152.55

Calcein AM/PI Double Staining Kit

E-CK-A354-each each Ask for price
Description: Viability/Cytotoxicity

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 613
Description: Caspase Staining Kit for use activity in the research laboratory

Staining Trough+Lid Rectangular - EACH

MIC6008 EACH
EUR 31.05

Ponceau S Protein Staining Solution

PS003-B500ML 500 ml
EUR 25

Mouse to Mouse HRP Staining System

MTM003 400 Slides
EUR 249.43

Slides Staining Jar, with Ground - in

CG147-5X1NO 1 unit
EUR 124
Description: Slides Staining Jar, with Ground - in

Preloading Fluorescent Staining Solution

abx460011-1096tests 10 × 96 tests Ask for price

Preloading Fluorescent Staining Solution

abx460011-596tests 5 × 96 tests Ask for price

Preloading Fluorescent Staining Solution

abx460011-96tests 96 tests
EUR 250

Staining Jar PP + Lid 10 Slide - EACH

MIC6002 EACH
EUR 75.6

Coomassie Blue Fast Staining Solution

AR0170 100mL(for 10 assays to stain gels of 5 x 8.5cm)
EUR 96

Alkaline Phosphatase Red Staining kit

TBS2080 50 tests
EUR 89

Coomassie Blue Fast Staining Solution

EZWB15-500mL 500mL
EUR 14.4

Calcein-AM/PI Double Staining Kit

E-CK-A354-100Assays 100 Assays
EUR 100

Calcein-AM/PI Double Staining Kit

E-CK-A354-2000Assays 2000 Assays
EUR 600

Calcein-AM/PI Double Staining Kit

E-CK-A354-500Assays 500 Assays
EUR 300

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 610
Description: Detects activated Caspases-3 by FACS, FL or plate reader

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 490
Description: Detects activated Caspases-8 by FACS, FL or plate reader

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 610
Description: Detects activated Caspases-9 by FACS, FL or plate reader

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 285.6

Slides Staining Racks for 30 pieces,

CG149-5X1NO 1 unit
EUR 124
Description: Slides Staining Racks for 30 pieces,

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