The identification of circulating biomarkers that closely correlate with Parkinson’s Disease (PD) has failed several times in the past. Nevertheless, in this pilot study, a translational approach was conducted, allowing the evaluation of the plasma levels of two mitochondrial-related proteins, whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients from matched controls.
The proposed translational approach was initiated by the analysis of secretomes from cells cultured under control or well-defined oxidative stress conditions, followed by the identification of proteins related to PD pathologic mechanisms that were altered between the two states. This pipeline was further translated into the analysis of undepleted plasma samples from 28 control and 31 PD patients.From the secretome analysis, several mitochondria-related proteins were found to be differentially released between control and stress conditions and to be able to distinguish the two secretomes.
Similarly, two mitochondrial-related proteins were found to be significantly changed in a PD cohort compared to matched controls. Moreover, a linear discriminant model with potential diagnostic value to discriminate PD patients was obtained using the combination of these two proteins. Both proteins are associated with apoptotic mitochondrial changes, which may correspond to potential indicators of cell death. Moreover, one of these proteins, the VPS35 protein, was reported in plasma for the first time, and its quantification was only possible due to its previous identification in the secretome analysis.

In this work, an adaptation of a translational pipeline for biomarker selection was presented and transposed to neurological diseases, in the present case Parkinson’s Disease. The novelty and success of this pilot study may arise from the combination of: i) a translational research pipeline, where plasma samples are interrogated using knowledge previously obtained from the evaluation of cells’ secretome under oxidative stress; ii) the combined used of statistical analysis and an informed selection of candidates based on their link with relevant disease mechanisms, and iii) the use of SWATH-MS, an untargeted MS method that allows a complete record of the analyzed samples and a targeted data extraction of the quantitative values of proteins previously identified.
Mouse to Mouse HRP (DAB) Staining System |
|||
MTM001 | ScyTek Laboratories | 70 Slides | EUR 157 |
SensiTek HRP Anti-Mouse (DAB) Staining System |
|||
AEA080 | ScyTek Laboratories | 70 Slides | EUR 119 |
SensiTek HRP Anti-Rabbit (DAB) Staining System |
|||
AEI080 | ScyTek Laboratories | 70 Slides | EUR 119 |
SensiTek HRP Anti-Polyvalent (DAB) Staining System |
|||
AEM080 | ScyTek Laboratories | 70 Slides | EUR 127 |
UltraTek HRP Anti-Mouse (DAB) Staining System |
|||
AKA080 | ScyTek Laboratories | 70 Slides | EUR 127 |
UltraTek HRP Anti-Rabbit (DAB) Staining System |
|||
AKC080 | ScyTek Laboratories | 70 Slides | EUR 127 |
UltraTek HRP Anti-Polyvalent (DAB) Staining System |
|||
AMF080 | ScyTek Laboratories | 70 Slides | EUR 138 |
Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml |
|||
NB319-SBB | Innovex | 30 ml | EUR 369 |
DAB |
|||
HY-15912 | MedChemExpress | 500mg | EUR 108 |
Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW |
|||
NB319-BB70 | Innovex | 70 ml | EUR 485 |
Fmoc-Dab[Fmoc-Dab(Boc)]-OH |
|||
5-04727 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab[Fmoc-Dab(Boc)]-OH |
|||
5-04728 | CHI Scientific | 25g | Ask for price |
STAT-Q Rabbit DAB KIT for staining all Rabbit antibodies, 350 plus slides |
|||
NB314KLRD-20 | Innovex | 20 ml kit | EUR 717 |
DAB Tablet |
|||
D3225-020 | GenDepot | 20TB | EUR 142 |
DAB Tablet |
|||
D3225-050 | GenDepot | 50TB | EUR 246 |
STAT-Q Goat DAB KIT for staining all Goat primary antibodies, 350 plus slides |
|||
NB314KLGD-20 | Innovex | 20 ml kit | EUR 717 |
DAB Chromogen Concentrate |
|||
D3226-002 | GenDepot | 20ml | EUR 161 |
DAB Chromogen Concentrate |
|||
D3226-005 | GenDepot | 50ml | EUR 334 |
Boc-Dab-OH |
|||
A-3215.0001 | Bachem | 1.0g | EUR 126 |
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6] |
Boc-Dab-OH |
|||
A-3215.0005 | Bachem | 5.0g | EUR 418 |
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6] |
Boc-Dab-OH |
|||
A-3215.0025 | Bachem | 25.0g | EUR 1578 |
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6] |
H-Dab-OH·HCl |
|||
5-04703 | CHI Scientific | 5g | Ask for price |
H-Dab-OH·HCl |
|||
5-04704 | CHI Scientific | 25g | Ask for price |
Boc-Dab-OH |
|||
5-04711 | CHI Scientific | 5g | Ask for price |
Boc-Dab-OH |
|||
5-04712 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab-OAll·HCl |
|||
5-04720 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab-OH·HCl |
|||
5-04721 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab-OH·HCl |
|||
5-04722 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab-OH |
|||
5-04735 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab-OH |
|||
5-04736 | CHI Scientific | 5g | Ask for price |
DAB Substrate kit |
|||
30015 | Biotium | 1Kit | EUR 143 |
Description: Minimum order quantity: 1 unit of 1Kit |
DAB Chromogen Kit |
|||
abx097197-1Kit | Abbexa | 1 Kit | EUR 272 |
H-Dab-OH |
|||
F-3050.0001 | Bachem | 1.0g | EUR 115 |
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6] |
H-Dab-OH |
|||
F-3050.0005 | Bachem | 5.0g | EUR 225 |
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6] |
H-Dab-OH |
|||
F-3050.0025 | Bachem | 25.0g | EUR 803 |
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6] |
Z-Dab-OH |
|||
C-3705.0001 | Bachem | 1.0g | EUR 115 |
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6] |
Z-Dab-OH |
|||
C-3705.0005 | Bachem | 5.0g | EUR 273 |
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6] |
Z-Dab-OH |
|||
C-3705.0025 | Bachem | 25.0g | EUR 998 |
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6] |
Fmoc-Dab-OH |
|||
B-2300.0001 | Bachem | 1.0g | EUR 151 |
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7] |
Fmoc-Dab-OH |
|||
B-2300.0005 | Bachem | 5.0g | EUR 515 |
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7] |
DAB Chromogen, 4ml |
|||
NB314D | Innovex | 4 ml | EUR 246 |
DL-Dab.2HCl |
|||
A6727-100000 | ApexBio | 100 g | EUR 569 |
Description: DL-Dab.2HCl |
DL-Dab.2HCl |
|||
A6727-25000 | ApexBio | 25 g | EUR 309 |
Description: DL-Dab.2HCl |
DL-Dab.2HCl |
|||
A6727-5000 | ApexBio | 5 g | EUR 180 |
Description: DL-Dab.2HCl |
DAB Chromogen Concentrate |
|||
ACB030 | ScyTek Laboratories | 30 ml | EUR 119 |
DAB Chromogen Concentrate |
|||
ACB060 | ScyTek Laboratories | 60 ml | EUR 165 |
DAB Chromogen Concentrate |
|||
ACB125 | ScyTek Laboratories | 125 ml | EUR 245 |
DAB Chromogen Concentrate |
|||
ACB500 | ScyTek Laboratories | 500 ml | EUR 671 |
DAB Chromogen Concentrate |
|||
ACB999 | ScyTek Laboratories | 1000 ml | EUR 1159 |
DAB Substrate Buffer |
|||
ACC500 | ScyTek Laboratories | 500 ml | EUR 72 |
DAB Substrate Buffer |
|||
ACC999 | ScyTek Laboratories | 1000 ml | EUR 80 |
DAB Enhancer Solution |
|||
ACM030 | ScyTek Laboratories | 30 ml | EUR 71 |
DAB Away Kit |
|||
ACP001 | ScyTek Laboratories | 500 ml | EUR 98 |
DAB Differentiating Solution |
|||
DDS500 | ScyTek Laboratories | 500 ml | EUR 109 |
?-Galactosidase Staining Kit |
|||
AKR-100 | Cell Biolabs | 1 kit | EUR 432 |
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol. |
DAPI Staining Kit |
|||
abx097206-1Kit | Abbexa | 1 Kit | EUR 230 |
PI Staining Kit |
|||
abx097208-1Kit | Abbexa | 1 Kit | EUR 230 |
Sensitive DAB Stain Kit |
|||
PW023 | Bio Basic | 5Preps, 5prep | EUR 69.14 |
H-Dab(Boc)-OH |
|||
A-3305.0001 | Bachem | 1.0g | EUR 200 |
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7] |
H-Dab(Boc)-OH |
|||
A-3305.0005 | Bachem | 5.0g | EUR 696 |
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7] |
Boc-Dab(Fmoc)-OH |
|||
A-3520.0001 | Bachem | 1.0g | EUR 225 |
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5] |
Boc-Dab(Fmoc)-OH |
|||
A-3520.0005 | Bachem | 5.0g | EUR 780 |
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5] |
Boc-Dab(Aloc)-OH |
|||
A-4125.0001 | Bachem | 1.0g | EUR 309 |
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8] |
Boc-Dab(Aloc)-OH |
|||
A-4125.0005 | Bachem | 5.0g | EUR 1143 |
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8] |
Boc-D-Dab-OH |
|||
A-4215.0001 | Bachem | 1.0g | EUR 418 |
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9] |
Boc-D-Dab-OH |
|||
A-4215.0005 | Bachem | 5.0g | EUR 1554 |
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9] |
Boc-Dab-OtBu · HCl |
|||
A-4415.0001 | Bachem | 1.0g | EUR 309 |
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net |
Boc-Dab-OtBu · HCl |
|||
A-4415.0005 | Bachem | 5.0g | EUR 1143 |
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net |
H-Dab(Z)-OH |
|||
5-04707 | CHI Scientific | 5g | Ask for price |
H-Dab(Z)-OH |
|||
5-04708 | CHI Scientific | 25g | Ask for price |
Boc-Dab(Boc)-OH·DCHA |
|||
5-04713 | CHI Scientific | 1g | Ask for price |
Boc-Dab(Boc)-OH·DCHA |
|||
5-04714 | CHI Scientific | 5g | Ask for price |
Boc-Dab(Fmoc)-OH |
|||
5-04715 | CHI Scientific | 5g | Ask for price |
Boc-Dab(Fmoc)-OH |
|||
5-04716 | CHI Scientific | 25g | Ask for price |
Boc-Dab(Z)-OH·DCHA |
|||
5-04717 | CHI Scientific | 5g | Ask for price |
Boc-Dab(Z)-OH·DCHA |
|||
5-04718 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab(Ac)-OH |
|||
5-04723 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(Ac)-OH |
|||
5-04724 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab(Boc)-OH |
|||
5-04725 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(Boc)-OH |
|||
5-04726 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab(Fmoc)-OH |
|||
5-04729 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(Fmoc)-OH |
|||
5-04730 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(MMt)-OH |
|||
5-04731 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(MMt)-OH |
|||
5-04732 | CHI Scientific | 25g | Ask for price |
Fmoc-Dab(Mtt)-OH |
|||
5-04733 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(Mtt)-OH |
|||
5-04734 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(Dde)-OH |
|||
5-04739 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(Dde)-OH |
|||
5-04740 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(ivDde)-OH |
|||
5-04741 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(ivDde)-OH |
|||
5-04742 | CHI Scientific | 5g | Ask for price |
Fmoc-Dab(Z)-OH |
|||
5-04743 | CHI Scientific | 1g | Ask for price |
Fmoc-Dab(Z)-OH |
|||
5-04744 | CHI Scientific | 5g | Ask for price |
Boc-D-Dab-OH |
|||
5-04745 | CHI Scientific | 1g | Ask for price |
Boc-D-Dab-OH |
|||
5-04746 | CHI Scientific | 5g | Ask for price |
Fmoc-D-Dab-OH |
|||
5-04751 | CHI Scientific | 1g | Ask for price |
Fmoc-D-Dab-OH |
|||
5-04752 | CHI Scientific | 5g | Ask for price |