A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkers.

A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.

The identification of circulating biomarkers that closely correlate with Parkinson’s Disease (PD) has failed several times in the past. Nevertheless, in this pilot study, a translational approach was conducted, allowing the evaluation of the plasma levels of two mitochondrial-related proteins, whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients from matched controls.

The proposed translational approach was initiated by the analysis of secretomes from cells cultured under control or well-defined oxidative stress conditions, followed by the identification of proteins related to PD pathologic mechanisms that were altered between the two states. This pipeline was further translated into the analysis of undepleted plasma samples from 28 control and 31 PD patients.From the secretome analysis, several mitochondria-related proteins were found to be differentially released between control and stress conditions and to be able to distinguish the two secretomes.

Similarly, two mitochondrial-related proteins were found to be significantly changed in a PD cohort compared to matched controls. Moreover, a linear discriminant model with potential diagnostic value to discriminate PD patients was obtained using the combination of these two proteins. Both proteins are associated with apoptotic mitochondrial changes, which may correspond to potential indicators of cell death. Moreover, one of these proteins, the VPS35 protein, was reported in plasma for the first time, and its quantification was only possible due to its previous identification in the secretome analysis.

A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkers.
A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.

In this work, an adaptation of a translational pipeline for biomarker selection was presented and transposed to neurological diseases, in the present case Parkinson’s Disease. The novelty and success of this pilot study may arise from the combination of: i) a translational research pipeline, where plasma samples are interrogated using knowledge previously obtained from the evaluation of cells’ secretome under oxidative stress; ii) the combined used of statistical analysis and an informed selection of candidates based on their link with relevant disease mechanisms, and iii) the use of SWATH-MS, an untargeted MS method that allows a complete record of the analyzed samples and a targeted data extraction of the quantitative values of proteins previously identified.

UltraTek HRP Anti-Mouse (DAB) Staining System

AKA080 70 Slides
EUR 152.4

UltraTek HRP Anti-Rabbit (DAB) Staining System

AKC080 70 Slides
EUR 152.4

UltraTek HRP Anti-Polyvalent (DAB) Staining System

AMF080 70 Slides
EUR 165.6

SensiTek HRP Anti-Mouse (DAB) Staining System

AEA080 70 Slides
EUR 142.8

SensiTek HRP Anti-Polyvalent (DAB) Staining System

AEM080 70 Slides
EUR 152.4

SensiTek HRP Anti-Rabbit (DAB) Staining System

AEI080 70 Slides
EUR 142.8

Mouse to Mouse HRP (DAB) Staining System

MTM001 70 Slides
EUR 188.4

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml

NB319-SBB 30 ml
EUR 442.8

DAB

HY-15912 500mg
EUR 129.6

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW

NB319-BB70 70 ml
EUR 582

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04727 5g Ask for price

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04728 25g Ask for price

STAT-Q Rabbit DAB KIT for staining all Rabbit antibodies, 350 plus slides

NB314KLRD-20 20 ml kit
EUR 860.4

DAB Tablet

D3225-020 20TB
EUR 170.4

DAB Tablet

D3225-050 50TB
EUR 295.2

STAT-Q Goat DAB KIT for staining all Goat primary antibodies, 350 plus slides

NB314KLGD-20 20 ml kit
EUR 860.4

H-Dab-OH·HCl

5-04703 5g Ask for price

H-Dab-OH·HCl

5-04704 25g Ask for price

Boc-Dab-OH

5-04711 5g Ask for price

Boc-Dab-OH

5-04712 25g Ask for price

Fmoc-Dab-OAll·HCl

5-04720 5g Ask for price

Fmoc-Dab-OH·HCl

5-04721 5g Ask for price

Fmoc-Dab-OH·HCl

5-04722 25g Ask for price

Fmoc-Dab-OH

5-04735 1g Ask for price

Fmoc-Dab-OH

5-04736 5g Ask for price

DAB Substrate kit

30015 1Kit
EUR 171.6
Description: Minimum order quantity: 1 unit of 1Kit

DAB Chromogen Kit

abx097197-1Kit 1 Kit
EUR 326.4

DL-Dab.2HCl

A6727-100000 100 g
EUR 682.8
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-25000 25 g
EUR 370.8
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-5000 5 g
EUR 216
Description: DL-Dab.2HCl

Boc-Dab-OH

A-3215.0001 1.0g
EUR 151.2
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0005 5.0g
EUR 501.6
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0025 25.0g
EUR 1893.6
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Fmoc-Dab-OH

B-2300.0001 1.0g
EUR 181.2
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

Fmoc-Dab-OH

B-2300.0005 5.0g
EUR 618
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

DAB Chromogen Concentrate

D3226-002 20ml
EUR 193.2

DAB Chromogen Concentrate

D3226-005 50ml
EUR 400.8

DAB Differentiating Solution

DDS500 500 ml
EUR 130.8

Z-Dab-OH

C-3705.0001 1.0g
EUR 138
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0005 5.0g
EUR 327.6
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0025 25.0g
EUR 1197.6
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

DAB Chromogen Concentrate

ACB030 30 ml
EUR 142.8

DAB Chromogen Concentrate

ACB060 60 ml
EUR 198

DAB Chromogen Concentrate

ACB125 125 ml
EUR 294

DAB Chromogen Concentrate

ACB500 500 ml
EUR 805.2

DAB Chromogen Concentrate

ACB999 1000 ml
EUR 1390.8

DAB Substrate Buffer

ACC500 500 ml
EUR 86.4

DAB Substrate Buffer

ACC999 1000 ml
EUR 96

DAB Enhancer Solution

ACM030 30 ml
EUR 85.2

DAB Away Kit

ACP001 500 ml
EUR 117.6

DAB Chromogen, 4ml

NB314D 4 ml
EUR 295.2

H-Dab-OH

F-3050.0001 1.0g
EUR 138
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0005 5.0g
EUR 270
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0025 25.0g
EUR 963.6
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 518.4
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

H-Dab(Z)-OH

5-04707 5g Ask for price

H-Dab(Z)-OH

5-04708 25g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04713 1g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04714 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04715 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04716 25g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04717 5g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04718 25g Ask for price

Fmoc-Dab(Ac)-OH

5-04723 5g Ask for price

Fmoc-Dab(Ac)-OH

5-04724 25g Ask for price

Fmoc-Dab(Boc)-OH

5-04725 5g Ask for price

Fmoc-Dab(Boc)-OH

5-04726 25g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04729 1g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04730 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04731 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04732 25g Ask for price

Fmoc-Dab(Mtt)-OH

5-04733 1g Ask for price

Fmoc-Dab(Mtt)-OH

5-04734 5g Ask for price

Fmoc-Dab(Dde)-OH

5-04739 1g Ask for price

Fmoc-Dab(Dde)-OH

5-04740 5g Ask for price

Fmoc-Dab(ivDde)-OH

5-04741 1g Ask for price

Fmoc-Dab(ivDde)-OH

5-04742 5g Ask for price

Fmoc-Dab(Z)-OH

5-04743 1g Ask for price

Fmoc-Dab(Z)-OH

5-04744 5g Ask for price

Boc-D-Dab-OH

5-04745 1g Ask for price

Boc-D-Dab-OH

5-04746 5g Ask for price

Fmoc-D-Dab-OH

5-04751 1g Ask for price

Fmoc-D-Dab-OH

5-04752 5g Ask for price

10-DAB (10-Deacetylbaccatin)

A4395-25 25 mg
EUR 616.8
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-5 5 mg
EUR 199.2
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-5.1 10 mM (in 1mL DMSO)
EUR 351.6
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

10-DAB (10-Deacetylbaccatin)

A4395-S Evaluation Sample
EUR 97.2
Description: 10-DAB (also known as 10-Deacetylbaccatin) is an intermediate used for the preparation of taxol, an anti-leukemic and tumor-inhibiting agent isolated from the inner bark of the Pacific yew tree Taxus brevifolia as well as other species of the genus Taxus.

Fmoc-Dab(Fmoc)-Oh

abx188721-250g 250 g
EUR 2064

H-Dab(Boc)-OH

A-3305.0001 1.0g
EUR 240
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

H-Dab(Boc)-OH

A-3305.0005 5.0g
EUR 835.2
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

Boc-Dab(Fmoc)-OH

A-3520.0001 1.0g
EUR 270
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Fmoc)-OH

A-3520.0005 5.0g
EUR 936
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Aloc)-OH

A-4125.0001 1.0g
EUR 370.8
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-Dab(Aloc)-OH

A-4125.0005 5.0g
EUR 1371.6
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-D-Dab-OH

A-4215.0001 1.0g
EUR 501.6
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-D-Dab-OH

A-4215.0005 5.0g
EUR 1864.8
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-Dab-OtBu · HCl

A-4415.0001 1.0g
EUR 370.8
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Boc-Dab-OtBu · HCl

A-4415.0005 5.0g
EUR 1371.6
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Fmoc-Dab(Boc)-OH

B-1800.0001 1.0g
EUR 240
Description: Sum Formula: C24H28N2O6; CAS# [125238-99-5]

Fmoc-Dab(Boc)-OH

B-1800.0005 5.0g
EUR 819.6
Description: Sum Formula: C24H28N2O6; CAS# [125238-99-5]

Fmoc-Dab(Fmoc)-OH

B-2270.0001 1.0g
EUR 283.2
Description: Sum Formula: C34H30N2O6; CAS# [201473-83-8]

Fmoc-Dab(Fmoc)-OH

B-2270.0005 5.0g
EUR 1039.2
Description: Sum Formula: C34H30N2O6; CAS# [201473-83-8]

Fmoc-D-Dab-OH

B-2365.0001 1.0g
EUR 501.6
Description: Sum Formula: C19H20N2O4; CAS# [201484-12-0]

Fmoc-D-Dab-OH

B-2365.0005 5.0g
EUR 1864.8
Description: Sum Formula: C19H20N2O4; CAS# [201484-12-0]

Fmoc-Dab(Aloc)-OH

B-2850.0001 1.0g
EUR 356.4
Description: Sum Formula: C23H24N2O6; CAS# [204316-32-5]

Fmoc-Dab(Aloc)-OH

B-2850.0005 5.0g
EUR 1327.2
Description: Sum Formula: C23H24N2O6; CAS# [204316-32-5]

Fmoc-Dab(Z)-OH

B-3250.0001 1.0g
EUR 313.2
Description: Sum Formula: C27H26N2O6; CAS# [252049-08-4]

Fmoc-Dab(Z)-OH

B-3250.0005 5.0g
EUR 1110
Description: Sum Formula: C27H26N2O6; CAS# [252049-08-4]

Fmoc-Dab(ivDde)-OH

B-4065.0001 1.0g
EUR 356.4
Description: Sum Formula: C32H38N2O6; CAS# [607366-21-2]

Fmoc-Dab(ivDde)-OH

B-4065.0005 5.0g
EUR 1311.6
Description: Sum Formula: C32H38N2O6; CAS# [607366-21-2]

DAB (Post) Enhancing Solution

DES500 500 ml
EUR 105.6

DAB (Post) Enhancing Solution

DES999 1000 ml
EUR 130.8

Z-Dab(Z)-OH

C-3690.0001 1.0g
EUR 240
Description: Sum Formula: C20H22N2O6; CAS# [55478-23-4]

Z-Dab(Z)-OH

C-3690.0005 5.0g
EUR 835.2
Description: Sum Formula: C20H22N2O6; CAS# [55478-23-4]

Z-D-Dab-OH

C-3770.0001 1.0g
EUR 270
Description: Sum Formula: C12H16N2O4; CAS# [70882-66-5]

Z-D-Dab-OH

C-3770.0005 5.0g
EUR 950.4
Description: Sum Formula: C12H16N2O4; CAS# [70882-66-5]

DAB Chromogen/Substrate Kit

ACH500 500 Slides
EUR 102

DAB Substrate (High Contrast)

ACU250 250 ml
EUR 102

DAB Substrate (High Contrast)

ACU500 500 ml
EUR 117.6

DAB Substrate (High Contrast)

ACU999 1000 ml
EUR 142.8

DAB Substrate buffer, 70ml

NB314SB 70 ml
EUR 331.2

Ac-Dab(Boc)-OH

F-1020.0250 250.0mg
EUR 283.2
Description: Sum Formula: C11H20N2O5; CAS# [201351-32-8]

Ac-Dab(Boc)-OH

F-1020.1000 1.0g
EUR 792
Description: Sum Formula: C11H20N2O5; CAS# [201351-32-8]

H-D-Dab-OH

F-3055.0001 1.0g
EUR 270
Description: Sum Formula: C4H10N2O2; CAS# [26908-94-1] net

H-D-Dab-OH

F-3055.0005 5.0g
EUR 950.4
Description: Sum Formula: C4H10N2O2; CAS# [26908-94-1] net

Sensitive DAB Stain Kit

PW023 5Preps, 5prep
EUR 82.97

STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 1000 plus slides (large size kit)

NB314KLD 1000 slides
EUR 1236

STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 350 slides kit (small size Kit)

NB314KLD-20 300 slide kit
EUR 818.4

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 444
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 390.96

PI / RNase Staining Buffer

20-abx090619
  • EUR 326.40
  • EUR 276.00
  • 100 tests
  • 50 tests

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Human Histochemistry Staining Kit

20-abx299039
  • EUR 1045.20
  • EUR 1462.80
  • EUR 678.00
  • 150 tests
  • 500 tests
  • 50 tests

Ponceau S Staining Solution

B7778-100000 100 g
EUR 195.6

Ponceau S Staining Solution

B7778-50000 50 g
EUR 151.2

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 211.2

Beta-Galactosidase Staining Kit

K802-250
EUR 352.8

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 69.6

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 351.6

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Disposable Slide Staining Trays

MIC5070 PK4
EUR 71.82

Staining Trough+Lid Rectangular

MIC6008 EACH
EUR 23.03

Nalgene Box Staining 13x13x5cm

BOX1040 EACH
EUR 42.18

Boc-D-Dab(Fmoc)-OH

5-04747 5g Ask for price

Boc-D-Dab(Fmoc)-OH

5-04748 25g Ask for price

Boc-D-Dab(Z)-OH·DCHA

5-04749 5g Ask for price

Boc-D-Dab(Z)-OH·DCHA

5-04750 25g Ask for price

Fmoc-D-Dab(Alloc)-OH

5-04753 1g Ask for price

Fmoc-D-Dab(Alloc)-OH

5-04754 5g Ask for price

Fmoc-D-Dab(Boc)-OH

5-04755 1g Ask for price

Fmoc-D-Dab(Boc)-OH

5-04756 5g Ask for price

Fmoc-D-Dab(Dde)-OH

5-04757 1g Ask for price

Fmoc-D-Dab(Dde)-OH

5-04758 5g Ask for price

Fmoc-D-Dab(MMt)-OH

5-04759 5g Ask for price

Fmoc-D-Dab(MMt)-OH

5-04760 25g Ask for price

Fmoc-D-Dab(Z)-OH

5-04761 250mg Ask for price

Fmoc-D-Dab(Z)-OH

5-04762 1g Ask for price

H-D-Dab-OH·2HCl

5-04763 5g Ask for price

H-D-Dab-OH·2HCl

5-04764 25g Ask for price

DAB Horseradish Peroxidase Chromogenic Kit

20-abx090660
  • EUR 276.00
  • EUR 226.80
  • 100 ml
  • 20 ml

H-D-Dab-OH.2HCl

A7493-1000 1 g
EUR 116.4
Description: H-D-Dab-OH?2HCl

H-D-Dab-OH.2HCl

A7493-25000 25 g
EUR 969.6
Description: H-D-Dab-OH?2HCl

H-D-Dab-OH.2HCl

A7493-5000 5 g
EUR 278.4
Description: H-D-Dab-OH?2HCl

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