The identification of circulating biomarkers that closely correlate with Parkinson’s Disease (PD) has failed several times in the past. Nevertheless, in this pilot study, a translational approach was conducted, allowing the evaluation of the plasma levels of two mitochondrial-related proteins, whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients from matched controls.
The proposed translational approach was initiated by the analysis of secretomes from cells cultured under control or well-defined oxidative stress conditions, followed by the identification of proteins related to PD pathologic mechanisms that were altered between the two states. This pipeline was further translated into the analysis of undepleted plasma samples from 28 control and 31 PD patients.From the secretome analysis, several mitochondria-related proteins were found to be differentially released between control and stress conditions and to be able to distinguish the two secretomes.
Similarly, two mitochondrial-related proteins were found to be significantly changed in a PD cohort compared to matched controls. Moreover, a linear discriminant model with potential diagnostic value to discriminate PD patients was obtained using the combination of these two proteins. Both proteins are associated with apoptotic mitochondrial changes, which may correspond to potential indicators of cell death. Moreover, one of these proteins, the VPS35 protein, was reported in plasma for the first time, and its quantification was only possible due to its previous identification in the secretome analysis.
A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.
In this work, an adaptation of a translational pipeline for biomarker selection was presented and transposed to neurological diseases, in the present case Parkinson’s Disease. The novelty and success of this pilot study may arise from the combination of: i) a translational research pipeline, where plasma samples are interrogated using knowledge previously obtained from the evaluation of cells’ secretome under oxidative stress; ii) the combined used of statistical analysis and an informed selection of candidates based on their link with relevant disease mechanisms, and iii) the use of SWATH-MS, an untargeted MS method that allows a complete record of the analyzed samples and a targeted data extraction of the quantitative values of proteins previously identified.
DAB Buffer, 10X For use with DAB Staining System for HRPO
MBS639268-50mL
MyBiosource
50mL
EUR 280
DAB Buffer, 10X For use with DAB Staining System for HRPO
MBS639268-5x500mL
MyBiosource
5x500mL
EUR 2090
UltraTek HRP Anti-Polyvalent (DAB) Staining System
AMF080
ScyTek Laboratories
70 Slides
EUR 70.71
SensiTek HRP Anti-Polyvalent (DAB) Staining System
AEM080
ScyTek Laboratories
70 Slides
EUR 61.72
DAB Enhancer for ampliying and darkening DAB stains in IHC staining, 60 ml (Large volume)
NB308
Innovex
60 ml
EUR 435
DAB Enhancer for ampliying and darkening DAB stains in IHC staining, 30 ml (Small volume)
NB308-30
Innovex
30 ml
EUR 285
DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO
MBS639197-100mL
MyBiosource
100mL
EUR 715
DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO
MBS639197-10mL
MyBiosource
10mL
EUR 300
DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO
MBS639197-25mL
MyBiosource
25mL
EUR 380
DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO
MBS639197-5x100mL
MyBiosource
5x100mL
EUR 3075
STAT-Q Rabbit DAB KIT for staining all Rabbit antibodies, 350 plus slides
NB314KLRD-20
Innovex
20 ml kit
EUR 645
STAT-Q Goat DAB KIT for staining all Goat primary antibodies, 350 plus slides
NB314KLGD-20
Innovex
20 ml kit
EUR 645
PI Staining Kit
abx097208-1Kit
Abbexa
1 Kit
EUR 276
HE staining Kit
MBS2567140-100mL
MyBiosource
100mL
EUR 255
HE staining Kit
MBS2567140-10mL
MyBiosource
10mL
EUR 120
HE staining Kit
MBS2567140-50mL
MyBiosource
50mL
EUR 195
HE staining Kit
MBS2567140-5x100mL
MyBiosource
5x100mL
EUR 1120
PI Staining Kit
MBS826722-01mL
MyBiosource
0.1mL
EUR 140
PI Staining Kit
MBS826722-5x01mL
MyBiosource
5x0.1mL
EUR 550
DAPI Staining Kit
abx097206-100l
Abbexa
100 µl
EUR 162.5
DAPI Staining Kit
abx097206-1Kit
Abbexa
1 Kit
EUR 276
DAPI Staining Kit
abx097206-1ml
Abbexa
1 ml
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DAPI Staining Kit
abx097206-200l
Abbexa
200 µl
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DAPI Staining Kit
MBS826728-01mL
MyBiosource
0.1mL
EUR 140
DAPI Staining Kit
MBS826728-5x01mL
MyBiosource
5x0.1mL
EUR 550
STAT-Q Rat Adsorbed DAB KIT for staining Mouse antibodies-on-Rat tissues 250 plus slides kit
NB314RAK-DAB
Innovex
200-250 slides
EUR 745
Silver Staining Kit
ML123-1KT
EWC Diagnostics
1 unit
EUR 201.16
Description: Silver Staining Kit
STAT-Q Mouse Adsorbed DAB KIT for staining Rat antibodies-on-Mouse tissues 250 plus slide kit
NB314MAK-DAB
Innovex
200-250 slides
EUR 695
DNA Staining Reagent
abx299713-100g
Abbexa
100 µg
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DNA Staining Reagent
abx299713-20g
Abbexa
20 µg
EUR 300
DNA Staining Reagent
abx299713-50g
Abbexa
50 µg
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Cell Staining Buffer
E16FXP005
EnoGene
500 ml
EUR 40
Description: Available in various conjugation types.
Cell Staining Buffer
MBS2567126-100mL
MyBiosource
100mL
EUR 90
Cell Staining Buffer
MBS2567126-200mL
MyBiosource
200mL
EUR 100
Cell Staining Buffer
MBS2567126-500mL
MyBiosource
500mL
EUR 125
Cell Staining Buffer
MBS2567126-5x500mL
MyBiosource
5x500mL
EUR 545
Cell Staining Buffer
MBS679239-500mL
MyBiosource
500mL
EUR 185
Cell Staining Buffer
MBS679239-5x500mL
MyBiosource
5x500mL
EUR 675
Cell Staining Buffer
MBS679240-100mL
MyBiosource
100mL
EUR 130
Cell Staining Buffer
MBS679240-5x100mL
MyBiosource
5x100mL
EUR 435
Cell Staining Buffer
MBS679241-250mL
MyBiosource
250mL
EUR 145
Cell Staining Buffer
MBS679241-5x250mL
MyBiosource
5x250mL
EUR 515
DAPI Staining Solution
MBS176580-10mL
MyBiosource
10mL
EUR 115
DAPI Staining Solution
MBS176580-50mL
MyBiosource
50mL
EUR 135
DAPI Staining Solution
MBS176580-5x50mL
MyBiosource
5x50mL
EUR 450
STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 350 slides kit (Small size Kit)
NB314KLD-20
Innovex
300 slide kit
EUR 655
Wright Staining Solution
K1182-100
ApexBio
100ml
EUR 40
Description: Cell Biology|Staining Solution
Wright Staining Solution
K1182-500
ApexBio
500ml
EUR 64
Description: Cell Biology|Staining Solution
STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 1000 plus slides (Large size kit)
NB314KLD
Innovex
1000 slides
EUR 895
ELVIS ID Staining Kit
SK-ELVIS-100
Quidel
100 Tests
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ELVIS ID Staining Kit
SK-ELVIS-1000
Quidel
1000 Tests
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ELVIS ID Staining Kit
SK-ELVIS-200
Quidel
200 Tests
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ELVIS ID Staining Kit
SK-ELVIS-500
Quidel
500 Tests
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Hoechst 33342 Staining Kit
abx097207-100l
Abbexa
100 µl
EUR 162.5
Hoechst 33342 Staining Kit
abx097207-1Kit
Abbexa
1 Kit
EUR 276
Hoechst 33342 Staining Kit
abx097207-1ml
Abbexa
1 ml
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Hoechst 33342 Staining Kit
abx097207-200l
Abbexa
200 µl
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Hoechst 33342 Staining Kit
MBS826727-01mL
MyBiosource
0.1mL
EUR 140
Hoechst 33342 Staining Kit
MBS826727-5x01mL
MyBiosource
5x0.1mL
EUR 550
DNA STAINING AGENT (1ML)
NOVELJUICE
Consort
each
EUR 80
Cartilage Staining Solution
MBS355813-100mL
MyBiosource
100mL
EUR 185
Cartilage Staining Solution
MBS355813-5x100mL
MyBiosource
5x100mL
EUR 530
Hematoxylin staining Buffer
MBS2567138-15mL
MyBiosource
15mL
EUR 125
Hematoxylin staining Buffer
MBS2567138-50mL
MyBiosource
50mL
EUR 190
Hematoxylin staining Buffer
MBS2567138-5mL
MyBiosource
5mL
EUR 95
Hematoxylin staining Buffer
MBS2567138-5x50mL
MyBiosource
5x50mL
EUR 815
Cell Cycle Staining Kit
KTA2020-100T
Abbkine
100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.
Cell Cycle Staining Kit
KTA2020-50T
Abbkine
50 T
EUR 69
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.
Cell Cycle Staining Kit
KTA2020-each
Abbkine
each
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Cell Cycle Staining Kit
MBS9718793-100Tests
MyBiosource
100Tests
EUR 225
Cell Cycle Staining Kit
MBS9718793-500Tests
MyBiosource
500Tests
EUR 355
Cell Cycle Staining Kit
MBS9718793-50Tests
MyBiosource
50Tests
EUR 195
Cell Cycle Staining Kit
MBS9718793-5x500Tests
MyBiosource
5x500Tests
EUR 1580
HISTOCHEMISTRY STAINING TRAY
AT-12
IBI Scientific
12 PLACE - BLACK LID
EUR 226.1
HISTOCHEMISTRY STAINING TRAY
AT-24
IBI Scientific
24 PLACE - BLACK LID
EUR 342.21
HISTOCHEMISTRY STAINING TRAY
ATC-12
IBI Scientific
12 PLACE - CLEAR LID
EUR 226.1
HISTOCHEMISTRY STAINING TRAY
ATC-24
IBI Scientific
24 PLACE - CLEAR LID
EUR 342.21
Exosome fluorescent staining
S104
101Bio
-
EUR 800
PI / RNase Staining Buffer
abx090619-100l
Abbexa
100 µl
EUR 162.5
PI / RNase Staining Buffer
20-abx090619
Abbexa
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PI / RNase Staining Buffer
abx090619-1ml
Abbexa
1 ml
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PI / RNase Staining Buffer
abx090619-200l
Abbexa
200 µl
EUR 200
Live NucRed staining kit
EGY069
EnoGene
>100T
EUR 210
PI / RNase Staining Buffer
MBS355225-100Tests
MyBiosource
100Tests
EUR 255
PI / RNase Staining Buffer
MBS355225-50Tests
MyBiosource
50Tests
EUR 215
PI / RNase Staining Buffer
MBS355225-5x100Tests
MyBiosource
5x100Tests
EUR 845
Live NucRed staining kit
MBS8584964-100Tests
MyBiosource
100Tests
EUR 310
Live NucRed staining kit
MBS8584964-5x100Tests
MyBiosource
5x100Tests
EUR 1405
7-AAD Staining Solution
MBS669126-200Tests
MyBiosource
200Tests
EUR 145
7-AAD Staining Solution
MBS669126-5x200Tests
MyBiosource
5x200Tests
EUR 655
Live NucGreen staining kit
EGY068
EnoGene
>100T
EUR 210
Dead NucGreen staining kit
EGY070
EnoGene
>100T
EUR 210
Live NucGreen staining kit
MBS8584963-100Tests
MyBiosource
100Tests
EUR 310
Live NucGreen staining kit
MBS8584963-5x100Tests
MyBiosource
5x100Tests
EUR 1405
Dead NucGreen staining kit
MBS8584965-100Tests
MyBiosource
100Tests
EUR 310
Dead NucGreen staining kit
MBS8584965-5x100Tests
MyBiosource
5x100Tests
EUR 1405
GEL STAINING/INCUBATION BOX
SB-10
IBI Scientific
LIGHT SHIELDING
EUR 94.44
Ponceau S Staining Solution
41640009-1
Bio-WORLD
500 mL
EUR 53.1
Ponceau S Staining Solution
41640009-2
Bio-WORLD
1 L
EUR 76.5
Ponceau S Staining Solution
B7778-100000
ApexBio
100 g
EUR 77.6
Description: Others|Reagents
Ponceau S Staining Solution
B7778-50000
ApexBio
50 g
EUR 52.8
Description: Others|Reagents
Giemsa Staining Solution (10X)
K1181-100
ApexBio
100ml
EUR 40
Description: Cell Biology|Staining Solution
Giemsa Staining Solution (10X)
K1181-500
ApexBio
500ml
EUR 64
Description: Cell Biology|Staining Solution
Ponceau S Staining Solution
MBS355693-100mL
MyBiosource
100mL
EUR 180
Ponceau S Staining Solution
MBS355693-5x100mL
MyBiosource
5x100mL
EUR 500
Sirius Red Staining Solution
MBS355815-100mL
MyBiosource
100mL
EUR 195
Sirius Red Staining Solution
MBS355815-5x100mL
MyBiosource
5x100mL
EUR 570
Ponceau S Staining Solution
PW005
Bio Basic
100ml
EUR 82.97
AO/PI Double Staining Kit
K2238-100
ApexBio
100T
EUR 40
Description: Cell Biology|Cell Apoptosis
AO/PI Double Staining Kit
K2238-500
ApexBio
500T
EUR 120
Description: Cell Biology|Cell Apoptosis
Neutral Red Staining Solution
K1185-100
ApexBio
100ml
EUR 40
Description: Cell Biology|Staining Solution
CellSurface FLOW Staining Kit
MBS668926-1Kit
MyBiosource
1Kit
EUR 200
CellSurface FLOW Staining Kit
MBS668926-5x1Kit
MyBiosource
5x1Kit
EUR 900
Dual- Color AP Staining Kit
AP100D-1
SBI
100 Assays
EUR 494.4
CometAssay Silver Staining Kit
4254-200-K
Biotechne
200 Tests
EUR 390.96
Methyl Green Staining Solution
abx090698-100l
Abbexa
100 µl
EUR 125
Methyl Green Staining Solution
abx090698-1ml
Abbexa
1 ml
Ask for price
Methyl Green Staining Solution
abx090698-200l
Abbexa
200 µl
EUR 187.5
β-Galactosidase Staining Kit
AKR-100
Cell Biolabs
1 kit
EUR 288
Description: Excepted Quantities
Methyl Green Staining Solution
K1180-100
ApexBio
100ml
EUR 40
Description: Cell Biology|Staining Solution
PI Viability Staining Solution
E16FXP002
EnoGene
150 tests
EUR 40
Description: Available in various conjugation types.
Methyl Green Staining Solution
MBS355816-100mL
MyBiosource
100mL
EUR 220
Methyl Green Staining Solution
MBS355816-10mL
MyBiosource
10mL
EUR 180
Methyl Green Staining Solution
MBS355816-5x100mL
MyBiosource
5x100mL
EUR 685
Live/Dead Cell Staining Kit
55R-1434
Fitzgerald
100 tests
EUR 500
Description: Live/Dead Cell Staining Kit for use in the research laboratory
Red-Color AP Staining Kit
AP100R-1
SBI
50 Assays
EUR 314.4
Beta Galactosidase Staining Kit
55R-1541
Fitzgerald
250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory
Lacto Fuchsin Staining Solution
97513
Sisco Laboratories
100 ml
EUR 3.01
Description: Part A
ProsiBlue Gel Staining Solution
P7300-050
GenDepot
500ml
EUR 102
STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides
NB330DB
Innovex
25 ml
EUR 595
Blue-Color AP Staining Kit
AP100B-1
SBI
50 Assays
EUR 203
Crystal Violet Staining Solution
K1184-100
ApexBio
100ml
EUR 40
Description: Cell Biology|Staining Solution
Crystal Violet Staining Solution
K1184-500
ApexBio
500ml
EUR 64
Description: Cell Biology|Staining Solution
Live-Dead Cell Staining Kit
GWB-AXR262
GenWay Biotech
100 stainings
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EZ-Quick Slide Staining Set
IW-2510
IHC World
-
EUR 435
Live-Dead Cell Staining Kit
K2081-100
ApexBio
100 staining
EUR 552
Live-Dead Cell Staining Kit
K2081-1000
ApexBio
1000T
EUR 124.8
Description: Tools Kit|Cell Staining Kit#Kits|Tools Kit#Kits
Live-Dead Cell Staining Kit
K2081-500
ApexBio
500T
EUR 76
Description: Tools Kit|Cell Staining Kit#Kits|Tools Kit#Kits
Live-Dead Cell Staining Kit
K501-100
Biovision
each
EUR 561.6
Human Histochemistry Staining Kit
20-abx299039
Abbexa
Ask for price
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50 tests
150 tests
500 tests
Beta-Galactosidase Staining Kit
GWB-AXR372
GenWay Biotech
200 assays.
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Beta-Galactosidase Staining Kit
K2182-250
ApexBio
250 assays
EUR 422.4
Description: Tools Kit|Molecular Biology Kit#Kits|Tools Kit#Kits