A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkers.

A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.

The identification of circulating biomarkers that closely correlate with Parkinson’s Disease (PD) has failed several times in the past. Nevertheless, in this pilot study, a translational approach was conducted, allowing the evaluation of the plasma levels of two mitochondrial-related proteins, whose combination leads to a robust model with potential diagnostic value to discriminate the PD patients from matched controls.

The proposed translational approach was initiated by the analysis of secretomes from cells cultured under control or well-defined oxidative stress conditions, followed by the identification of proteins related to PD pathologic mechanisms that were altered between the two states. This pipeline was further translated into the analysis of undepleted plasma samples from 28 control and 31 PD patients.From the secretome analysis, several mitochondria-related proteins were found to be differentially released between control and stress conditions and to be able to distinguish the two secretomes.

Similarly, two mitochondrial-related proteins were found to be significantly changed in a PD cohort compared to matched controls. Moreover, a linear discriminant model with potential diagnostic value to discriminate PD patients was obtained using the combination of these two proteins. Both proteins are associated with apoptotic mitochondrial changes, which may correspond to potential indicators of cell death. Moreover, one of these proteins, the VPS35 protein, was reported in plasma for the first time, and its quantification was only possible due to its previous identification in the secretome analysis.

A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkers.
A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson’s disease potential biomarkers.

In this work, an adaptation of a translational pipeline for biomarker selection was presented and transposed to neurological diseases, in the present case Parkinson’s Disease. The novelty and success of this pilot study may arise from the combination of: i) a translational research pipeline, where plasma samples are interrogated using knowledge previously obtained from the evaluation of cells’ secretome under oxidative stress; ii) the combined used of statistical analysis and an informed selection of candidates based on their link with relevant disease mechanisms, and iii) the use of SWATH-MS, an untargeted MS method that allows a complete record of the analyzed samples and a targeted data extraction of the quantitative values of proteins previously identified.

DAB Buffer, 10X For use with DAB Staining System for HRPO

MBS639268-50mL 50mL
EUR 280

DAB Buffer, 10X For use with DAB Staining System for HRPO

MBS639268-5x500mL 5x500mL
EUR 2090

SensiTek HRP Anti-Mouse (DAB) Staining System

AEA080 70 Slides
EUR 55.28

UltraTek HRP Anti-Mouse (DAB) Staining System

AKA080 70 Slides
EUR 61.72

SensiTek HRP Anti-Rabbit (DAB) Staining System

AEI080 70 Slides
EUR 55.28

UltraTek HRP Anti-Rabbit (DAB) Staining System

AKC080 70 Slides
EUR 61.72

UltraTek HRP Anti-Polyvalent (DAB) Staining System

AMF080 70 Slides
EUR 70.71

SensiTek HRP Anti-Polyvalent (DAB) Staining System

AEM080 70 Slides
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STAT-Q Multivalent DAB KIT for staining Mouse, Rat

NB314KLD 1000 slides
EUR 895
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STAT-Q Multivalent DAB KIT for staining Mouse, Rat

NB314KLD-20 300 slide kit
EUR 595
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STAT-Q Goat DAB KIT for staining all Goat primary

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DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO

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DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO

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EUR 300

DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO

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DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO

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STAT Double Staining ragent for Double staining Mo

NB330DB 25 ml
EUR 595
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STAT Double Staining reagent for Double staining o

NB326DB 25 ml
EUR 595
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STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
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PI Staining Kit

abx097208-1Kit 1 Kit
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HE Staining Kit

E-IR-R117-100mL 100mL
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MBS826722-01mL 0.1mL
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abx097206-1Kit 1 Kit
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TRAP Staining Kit

KT-008 1 kit
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abx097206-100l 100 µl
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Cell Staining Buffer

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68015 250 ml
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Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

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Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

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PI / RNase Staining Buffer

MBS355225-100Tests 100Tests
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MBS355225-50Tests 50Tests
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7-AAD Staining Solution

MBS669126-200Tests 200Tests
EUR 145

7-AAD Staining Solution

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EUR 655

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EUR 210

Dead NucGreen staining kit

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Live NucGreen staining kit

MBS8584963-100Tests 100Tests
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Live NucGreen staining kit

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EUR 1405

Dead NucGreen staining kit

MBS8584965-100Tests 100Tests
EUR 310

Dead NucGreen staining kit

MBS8584965-5x100Tests 5x100Tests
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GEL STAINING/INCUBATION BOX

SB-10 LIGHT SHIELDING
EUR 94.44

Ponceau S Staining Solution

41640009-1 500 mL
EUR 40.13

Ponceau S Staining Solution

41640009-2 1 L
EUR 58.16

Ponceau S Staining Solution

B7778-100000 100 g
EUR 78
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

B7778-50000 50 g
EUR 53
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes

Ponceau S Staining Solution

14092 100 ml
EUR 13.13
Description: Part B

Ponceau S Staining Solution

EZWB08-1x-100mL 100mL
EUR 9.6

Ponceau S Staining Solution

EZWB08-1x-500mL 500mL
EUR 32.4

Giemsa Staining Solution (10X)

K1181-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

Giemsa Staining Solution (10X)

K1181-500 500ml
EUR 64
Description: Cell Biology|Staining Solution

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Ponceau S Staining Solution

MBS355693-100mL 100mL
EUR 180

Ponceau S Staining Solution

MBS355693-5x100mL 5x100mL
EUR 500

Sirius Red Staining Solution

MBS355815-100mL 100mL
EUR 195

Sirius Red Staining Solution

MBS355815-5x100mL 5x100mL
EUR 570

AO/PI Double Staining Kit

K2238-100 100T
EUR 40
Description: Cell Biology|Cell Apoptosis

AO/PI Double Staining Kit

K2238-500 500T
EUR 120
Description: Cell Biology|Cell Apoptosis

Neutral Red Staining Solution

K1185-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 101.58

CellSurface FLOW Staining Kit

MBS668926-1Kit 1Kit
EUR 200

CellSurface FLOW Staining Kit

MBS668926-5x1Kit 5x1Kit
EUR 900

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 390.96

PI Viability Staining Solution

E16FXP002 150 tests
EUR 40
Description: Available in various conjugation types.

β-Galactosidase Staining Kit

AKR-100 1 kit
EUR 345

Methyl Green Staining Solution

K1180-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

Methyl Green Staining Solution

abx090698-100l 100 µl
EUR 125

Methyl Green Staining Solution

abx090698-1ml 1 ml Ask for price

Methyl Green Staining Solution

abx090698-200l 200 µl
EUR 187.5

Methyl Green Staining Solution

MBS355816-100mL 100mL
EUR 220

Methyl Green Staining Solution

MBS355816-10mL 10mL
EUR 180

Methyl Green Staining Solution

MBS355816-5x100mL 5x100mL
EUR 685

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 500
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Lacto Fuchsin Staining Solution

97513 100 ml
EUR 3.01
Description: Part A

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 203

Crystal Violet Staining Solution

K1184-100 100ml
EUR 40
Description: Cell Biology|Staining Solution

Crystal Violet Staining Solution

K1184-500 500ml
EUR 64
Description: Cell Biology|Staining Solution

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

Live-Dead Cell Staining Kit

K2081-1000 1000T
EUR 112

Live-Dead Cell Staining Kit

K2081-500 500T
EUR 68

Live-Dead Cell Staining Kit

GWB-AXR262 100 stainings Ask for price

EZ-Quick Slide Staining Set

IW-2510 -
EUR 425

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Human Histochemistry Staining Kit

20-abx299039
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  • 50 tests

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 274

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